Continuous assessment of metabolic activity of mitochondria using resonance Raman microspectroscopy

Lalonde, Joshua W.; Noojin, Gary D.; Pope, Nathaniel J.; Powell, Samantha M.; Yakovlev, Vladislav V.; Denton, Michael L.

doi:10.1002/jbio.202000384

Title: Continuous assessment of metabolic activity of mitochondria using resonance Raman microspectroscopy

Journal Article · Wed Jan 13 00:00:00 EST 2021 · Journal of Biophotonics

DOI:https://doi.org/10.1002/jbio.202000384· OSTI ID:1781093

Lalonde, Joshua W. ^[1]; Noojin, Gary D. ^[2]; Pope, Nathaniel J. ^[2]; Powell, Samantha M. ^[3];

^[4]; Denton, Michael L. ^[5]

Texas A & M Univ., College Station, TX (United States); Joint Base San Antonio Fort Sam Houston, San Antonio, TX (United States); Consortium Research Fellows Program (CRFP), Washington, DC (United States)
Joint Base San Antonio Fort Sam Houston, San Antonio, TX (United States)
Joint Base San Antonio Fort Sam Houston, San Antonio, TX (United States). National Research Council Research Associateship Programs; Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Texas A & M Univ., College Station, TX (United States)
Joint Base San Antonio Fort Sam Houston, San Antonio, TX (United States). Air Force Research Lab. (AFRL)

Dysfunctional mitochondrial activity can lead to a variety of different diseases. As such, there exists a need to quantify changes in mitochondria function as it relates to these specific diseased states. Here, we present the use of resonance Raman (RR) spectroscopy as a tool to determine changes in isolated mitochondrial activity. RR spectroscopy, using 532 nm as the excitation source, specifically provides information on the reduction and oxidation (RedOx) state of cytochrome c, which is determined by the activity of protein complexes in the electron transport chain. In this model, injection of the substrate succinate into the mitochondrial sample is used to drive the electron transport chain, which causes a subsequent change in cytochrome c RedOx state. This change in RedOx state is tracked by RR spectroscopy. We report this tool gives real-time information on the rise and fall of the amount of reduced cytochrome c within the mitochondrial sample, providing a method for rapid assessment of mitochondrial metabolism that has broad applications in both basic science and medical research.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE; US Air Force Office of Scientific Research (AFOSR); US Army Research Laboratory (USARL); Cancer Prevention and Research Institute of Texas (CPRIT); USDOD Army Medical Research; National Institutes of Health (NIH); National Science Foundation (NSF)

Grant/Contract Number:: AC05-76RL01830; FA8650-14-D-6519; FA8650-19-C-6024; FA9550-20-1-0366; FA9550-20-1-0367; 19RHCOR067; FA9550-15-1-0517; FA9550-18-1-0141; W911NF-17-2-0144; RP180588; W81XWH2010777; 1R01GM127696-01; DBI-1455671; ECCS-1509268; CMMI-1826078

OSTI ID:: 1781093

Report Number(s):: PNNL-SA-156542

Journal Information:: Journal of Biophotonics, Vol. 14, Issue 4; ISSN 1864-063X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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