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Title: Open data set of live cyanobacterial cells imaged using an X-ray laser

Journal Article · · Scientific Data
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  1. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics
  2. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
  3. European X-ray Free-Electron Laser (XFEL), Hamburg (Germany)
  4. Arizona State Univ., Tempe, AZ (United States). Physics Dept.
  5. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Tor Vergata Univ. of Rome (Italy). Physics Dept. INFN
  6. European X-ray Free-Electron Laser (XFEL), Hamburg (Germany); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  7. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; Academy of Sciences of the Czech Republic, Prague (Czech Republic). Inst. of Physics. ELI beamlines
  8. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; Uppsala Univ. (Sweden). Dept. of Physics and Astronomy
  9. Univ. College London (United Kingdom). MRC Lab. for Molecular Cell Biology
  10. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; Jozef Stefan Inst. (IJS), Ljubljana (Slovenia). Center for Technology Transfer and Innovation
  11. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
  12. Univ. of Melbourne (Australia). ARC Centre of Excellence for Advanced Molecular Imaging. School of Physics
  13. Technische Univ. Berlin (Germany). Inst. fur Optik und Atomare Physik (IOAP)
  14. Synchrotron SOLEIL, Saint Aubin (France)
  15. Max Planck Advanced Study Group, Hamburg (Germany). Center for Free Electron Laser Science; Max Planck Inst. fuer Medizinische Forschung, Heidelberg (Germany)
  16. Max Planck Advanced Study Group, Hamburg (Germany). Center for Free Electron Laser Science; Max Planck Inst. fuer Kernphysik, Heidelberg (Germany)
  17. PNSensor GmbH, Munich (Germany)
  18. Max Planck Inst.Halbleiterlabor, Munich (Germany); Max Planck Inst. fuer Extraterrestrische Physik, Garching (Germany)
  19. Max Planck Inst. fuer Extraterrestrische Physik, Garching (Germany); Univ. Oldenburg (Germany). Ultrafast Coherent Dynamics Group
  20. National Univ. of Singapore (Singapore). Center for BioImaging Sciences
  21. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany)
  22. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; European X-ray Free-Electron Laser (XFEL), Hamburg (Germany)
+ Show Author Affiliations

Structural studies on living cells by conventional methods are limited to low resolution because radiation damage kills cells long before the necessary dose for high resolution can be delivered. X-ray free-electron lasers circumvent this problem by outrunning key damage processes with an ultra-short and extremely bright coherent X-ray pulse. Diffraction-before-destruction experiments provide high-resolution data from cells that are alive when the femtosecond X-ray pulse traverses the sample. This paper presents two data sets from micron-sized cyanobacteria obtained at the Linac Coherent Light Source, containing a total of 199,000 diffraction patterns. Utilizing this type of diffraction data will require the development of new analysis methods and algorithms for studying structure and structural variability in large populations of cells and to create abstract models. Such studies will allow us to understand living cells and populations of cells in new ways. New X-ray lasers, like the European XFEL, will produce billions of pulses per day, and could open new areas in structural sciences.

Research Organization:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-76SF00515
OSTI ID:
1624546
Journal Information:
Scientific Data, Vol. 3, Issue 1; ISSN 2052-4463
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (7)

Short-wavelength free-electron laser sources and science: a review text January 2017
Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source journal August 2016
The trickle before the torrent—diffraction data from X-ray lasers journal August 2016
The Potential of EuPRAXIA@SPARC_LAB for Radiation Based Techniques journal March 2019
A data set from flash X-ray imaging of carboxysomes journal August 2016
Short-wavelength free-electron laser sources and science: a review journal October 2017
Parameter optimization for 3D-reconstruction from XFEL diffraction patterns based on Fourier slice matching journal January 2019

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