A Ricin Forensic Profiling Approach Based on a Complex Set of Biomarkers
A forensic method for the retrospective determination of preparation methods used for illicit ricin toxin production was developed. The method was based on a complex set of biomarkers, including carbohydrates, fatty acids, seed storage proteins, in combination with data on ricin and Ricinus communis agglutinin. The analyses were performed on samples prepared from four castor bean plant (Ricinus communis) cultivars by four different sample preparation methods (PM1 – PM4) ranging from simple disintegration of the castor beans to multi-step preparation methods including different protein precipitation methods. Comprehensive analytical data was collected by use of a range of analytical methods and robust orthogonal partial least squares-discriminant analysis- models (OPLS-DA) were constructed based on the calibration set. By the use of a decision tree and two OPLS-DA models, the sample preparation methods of a test set of ricin preparations were determined. The model statistics of the two models were good and a 100% rate of correct predictions of the test set was achieved.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1490194
- Report Number(s):
- PNNL-SA-130526
- Journal Information:
- Talanta, Vol. 186, Issue C; ISSN 0039-9140
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
Biological Toxins as the Potential Tools for Bioterrorism
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journal | March 2019 |
Ricin and Ricinus communis in pharmacology and toxicology-from ancient use and “Papyrus Ebers” to modern perspectives and “poisonous plant of the year 2018”
|
journal | July 2019 |
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