A Ricin Forensic Profiling Approach Based on a Complex Set of Biomarkers

Fredriksson, Sten-Ake; Wunschel, David S.; Lindstrom, Susanne Wiklund; Nilsson, Calle; Wahl, Karen; Astot, Crister

doi:10.1016/J.TALANTA.2018.03.070

Title: A Ricin Forensic Profiling Approach Based on a Complex Set of Biomarkers

Journal Article · Wed Mar 28 00:00:00 EDT 2018 · Talanta

DOI:https://doi.org/10.1016/J.TALANTA.2018.03.070· OSTI ID:1430520

^[1]; Wunschel, David S. ^[2]; Lindstrom, Susanne Wiklund ^[1]; Nilsson, Calle ^[1]; Wahl, Karen ^[2]; Astot, Crister ^[1]

Swedish Defence Research Agency, Umea (Sweden)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

A forensic method for the retrospective determination of preparation methods used for illicit ricin toxin production was developed. The method was based on a complex set of biomarkers, including carbohydrates, fatty acids, seed storage proteins, in combination with data on ricin and Ricinus communis agglutinin. The analyses were performed on samples prepared from four castor bean plant (R. communis) cultivars by four different sample preparation methods (PM1 – PM4) ranging from simple disintegration of the castor beans to multi-step preparation methods including different protein precipitation methods. Comprehensive analytical data was collected by use of a range of analytical methods and robust orthogonal partial least squares-discriminant analysis- models (OPLS-DA) were constructed based on the calibration set. By the use of a decision tree and two OPLS-DA models, the sample preparation methods of test set samples were determined. The model statistics of the two models were good and a 100% rate of correct predictions of the test set was achieved.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC0576RL01830; AC06-76RLO; HSHQPM-11-X-00012/3

OSTI ID:: 1430520

Alternate ID(s):: OSTI ID: 1582890

Report Number(s):: PNNL-SA-130526; PII: S0039914018303102

Journal Information:: Talanta, Vol. 186; ISSN 0039-9140

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 9 works

Citation information provided by
Web of Science

References (22)

Ricinus communis Intoxications in Human and Veterinary Medicine—A Summary of Real Cases Worbs, Sylvia; Köhler, Kernt; Pauly, Diana Toxins, Vol. 3, Issue 10 https://doi.org/10.3390/toxins3101332	journal	October 2011
Different biological properties of the two constituent peptide chains of ricin a toxic protein inhibiting protein synthesis Olsnes, Sjur; Pihl, Alexander Biochemistry, Vol. 12, Issue 16 https://doi.org/10.1021/bi00740a028	journal	July 1973
Forensic Identification of Neat Ricin and of Ricin from Crude Castor Bean Extracts by Mass Spectrometry Fredriksson, Sten-Åke; Hulst, Albert G.; Artursson, Elisabeth Analytical Chemistry, Vol. 77, Issue 6 https://doi.org/10.1021/ac048756u	journal	February 2005
Characterization of Ricin Heterogeneity by Electrospray Mass Spectrometry, Capillary Electrophoresis, and Resonant Mirror Despeyroux, D.; Walker, N.; Pearce, M. Analytical Biochemistry, Vol. 279, Issue 1 https://doi.org/10.1006/abio.1999.4423	journal	March 2000
Purification and Physicochemical Properties of Ricins and Agglutinins from Ricinus communis Thomas, T. -S.; Steven, S. -L. European Journal of Biochemistry, Vol. 105, Issue 3 https://doi.org/10.1111/j.1432-1033.1980.tb04520.x	journal	April 1980
Integration of gas chromatography mass spectrometry methods for differentiating ricin preparation methods Wunschel, David S.; Melville, Angela M.; Ehrhardt, Christopher J. The Analyst, Vol. 137, Issue 9 https://doi.org/10.1039/c2an16186a	journal	January 2012
Analysis of active ricin and castor bean proteins in a ricin preparation, castor bean extract, and surface swabs from a public health investigation Schieltz, David M.; McGrath, Sara C.; McWilliams, Lisa G. Forensic Science International, Vol. 209, Issue 1-3 https://doi.org/10.1016/j.forsciint.2010.12.013	journal	June 2011
Identification of RIP-II Toxins by Affinity Enrichment, Enzymatic Digestion and LC-MS Fredriksson, Sten-Åke; Artursson, Elisabet; Bergström, Tomas Analytical Chemistry, Vol. 87, Issue 2 https://doi.org/10.1021/ac5032918	journal	December 2014
Detection of Acetone Processing of Castor Bean Mash for Forensic Investigation of Ricin Preparation Methods*: DETECTION OF ACETONE PROCESSING OF CASTOR BEAN MASH Kreuzer, Helen W.; Wahl, Jon H.; Metoyer, Candace N. Journal of Forensic Sciences, Vol. 55, Issue 4 https://doi.org/10.1111/j.1556-4029.2010.01334.x	journal	March 2010
Analysis of Carbohydrate and Fatty Acid Marker Abundance in Ricin Toxin Preparations for Forensic Information Colburn, Heather A.; Wunschel, David S.; Kreuzer, Helen W. Analytical Chemistry, Vol. 82, Issue 14 https://doi.org/10.1021/ac1006206	journal	July 2010
Forensic Determination of Ricin and the Alkaloid Marker Ricinine From Castor Bean Extracts Darby, Shauna M.; Miller, Mark L.; Allen, Ralph O. Journal of Forensic Sciences, Vol. 46, Issue 5 https://doi.org/10.1520/JFS15097J	journal	September 2001
De Novo Sequencing of RCB-1 to -3: Peptide Biomarkers from the Castor Bean Plant Ricinus communis Ovenden, Simon P. B.; Fredriksson, Sten-Åke; Bagas, Christina K. Analytical Chemistry, Vol. 81, Issue 10 https://doi.org/10.1021/ac900371y	journal	April 2009
Modern analytical techniques in metabolomics analysis Zhang, Aihua; Sun, Hui; Wang, Ping The Analyst, Vol. 137, Issue 2 https://doi.org/10.1039/C1AN15605E	journal	January 2012
Mass spectrometry-based plant metabolomics: Metabolite responses to abiotic stress: MASS SPECTROMETRY-BASED PLANT METABOLOMICS Jorge, Tiago F.; Rodrigues, João A.; Caldana, Camila Mass Spectrometry Reviews, Vol. 35, Issue 5 https://doi.org/10.1002/mas.21449	journal	January 2015
The application of multi-omics and systems biology to identify therapeutic targets in chronic kidney disease Cisek, Katryna; Krochmal, Magdalena; Klein, Julie Nephrology Dialysis Transplantation, Vol. 31, Issue 12 https://doi.org/10.1093/ndt/gfv364	journal	October 2015
Toward Systems Understanding of Leaf Senescence: An Integrated Multi-Omics Perspective on Leaf Senescence Research Kim, Jeongsik; Woo, Hye Ryun; Nam, Hong Gil Molecular Plant, Vol. 9, Issue 6 https://doi.org/10.1016/j.molp.2016.04.017	journal	June 2016
Multi -omics and metabolic modelling pipelines: Challenges and tools for systems microbiology Fondi, Marco; Liò, Pietro Microbiological Research, Vol. 171 https://doi.org/10.1016/j.micres.2015.01.003	journal	February 2015
Liquid Chromatography-Mass Spectrometry and Chemometric Analysis of Ricinus communis Extracts for Cultivar Identification: LC-MS and Chemometric Analysis of Ricinus communis Ovenden, Simon P. B.; Pigott, Eloise J.; Rochfort, Simone Phytochemical Analysis, Vol. 25, Issue 5 https://doi.org/10.1002/pca.2519	journal	April 2014
Mass Spectrometric Detection of Ricin and its Activity in Food and Clinical Samples Kalb, Suzanne R.; Barr, John R. Analytical Chemistry, Vol. 81, Issue 6 https://doi.org/10.1021/ac802769s	journal	February 2009
Detection of Intact Ricin in Crude and Purified Extracts from Castor Beans Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Brinkworth, Craig S.; Pigott, Eloise J.; Bourne, David J. Analytical Chemistry, Vol. 81, Issue 4 https://doi.org/10.1021/ac802240f	journal	January 2009
Castor oil: A vital industrial raw material Ogunniyi, D. Bioresource Technology, Vol. 97, Issue 9 https://doi.org/10.1016/j.biortech.2005.03.028	journal	June 2006
Metabolomic investigations of Ricinus communis for cultivar and provenance determination Pigott, Eloise J.; Roberts, Warren; Ovenden, Simon P. B. Metabolomics, Vol. 8, Issue 4 https://doi.org/10.1007/s11306-011-0355-7	journal	August 2011

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Biological Toxins as the Potential Tools for Bioterrorism Janik, Edyta; Ceremuga, Michal; Saluk-Bijak, Joanna International Journal of Molecular Sciences, Vol. 20, Issue 5 https://doi.org/10.3390/ijms20051181	journal	March 2019
Ricin and Ricinus communis in pharmacology and toxicology-from ancient use and “Papyrus Ebers” to modern perspectives and “poisonous plant of the year 2018” Franke, Heike; Scholl, Reinhold; Aigner, Achim Naunyn-Schmiedeberg's Archives of Pharmacology, Vol. 392, Issue 10 https://doi.org/10.1007/s00210-019-01691-6	journal	July 2019

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