Cross-referencing yeast genetics and mammalian genomes
- Johns Hopkins Medical School, Baltimore, MD (United States)
We have initiated a project that will systematically transfer information about yeast genes onto the genetic maps of mice and human beings. Rapidly expanding human EST data will serve as a source of candidate human homologs that will be repeatedly searched using yeast protein sequence queries. Search results will be automatically reported to participating labs. Human cDNA sequences from which the ESTs are derived will be mapped at high resolution in the human and mouse genomes. The comparative mapping information cross-references the genomic position of novel human cDNAs with functional information known about the cognate yeast genes. This should facilitate the initial identification of genes responsible for mammalian mutant phenotypes, including human disease. In addition, the identification of mammalian homologs of yeast genes provides reagents for determining evolutionary conservation and for performing direct experiments in multicellular eukaryotes to enhance study of the yeast protein`s function. For example, ESTs homologous to CDC27 and CDC16 were identified, and the corresponding cDNA clones were obtained from ATTC, completely sequenced, and mapped on human and mouse chromosomes. In addition, the CDC17hs cDNA has been used to raise antisera to the CDC27Hs protein and used in subcellular localization experiments and junctional studies in mammalian cells. We have received funding from the National Center for Human Genome Research to provide a community resource which will establish comprehensive cross-referencing among yeast, human, and mouse loci. The project is set up as a service and information on how to communicate with this effort will be provided.
- OSTI ID:
- 134632
- Report Number(s):
- CONF-941009-; ISSN 0002-9297; TRN: 95:005313-1369
- Journal Information:
- American Journal of Human Genetics, Vol. 55, Issue Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
- Country of Publication:
- United States
- Language:
- English
Similar Records
Isolation and characterization of the human and mouse homologues (SUPT4H and Supt4h) of the yeast SPT4 gene
Mitochondrial import of human and yeast fumarase in live mammalian cells: Retrograde translocation of the yeast enzyme is mainly caused by its poor targeting sequence
Related Subjects
COMPUTERS
INFORMATION SCIENCE
MANAGEMENT
LAW
MISCELLANEOUS
55 BIOLOGY AND MEDICINE
BASIC STUDIES
GENES
GENETIC MAPPING
BIOLOGICAL EVOLUTION
DNA-CLONING
INFORMATION SYSTEMS
INFORMATION CENTERS
RESOLUTION
DISEASES
MICE
COMPARATIVE EVALUATIONS
GENETICS
PROTEINS
DNA SEQUENCING
YEASTS
MAMMALS
PHENOTYPE
INFORMATION DISSEMINATION
DATA BASE MANAGEMENT