Isolation and characterization of the human and mouse homologues (SUPT4H and Supt4h) of the yeast SPT4 gene
- Univ. of Michigan Medical Center, Ann Arbor, MI (United States); and others
To study gene regulation mediated by chromatin in mammals, we isolated the human (SUPT4H) and murine (Supt4h) counterparts of the yeast gene encoding SPT4; the product of this gene presumably interacts with the products of the mammalian homologues (which we have also cloned) of yeast SPT5 and SPT6, thereby modulating chromatin formation and activity. We isolated two different sized human SUPT4H cDNA clones (1464 and 728 nt) and one murine Supt4h (688 nt) cDNA clone; all three encode the same 117-amino-acid protein with conservation of the zinc finger motif found in SPT4. Conservation of this zinc finger motif from yeast to mouse and human implies functional implies functional importance. Although the overall sequence homology at the DNA level between the human 728-nt transcript and the murine 688-nt transcript is only 78.4%, the DNA sequence homology is 97.7% within the coding region. At the protein level, the amino acid sequences of the translated murine Supt4h and the human SUPT4H gene products are identical. The likely functional copy of SUPT4H, which has at least two introns, maps to human chromosome 17, with candidate intronless pseudogenes on chromosomes 2, 12, and 20. But-tressing the hypothesis that this is a gene required constitutively, both the human SUPT4H transcripts and the murine Supt4h transcript are expressed widely, although not at equal levels (e.g., such as most histones), in all fetal and adult tissues that we examined. 18 refs., 5 figs.
- OSTI ID:
- 466685
- Journal Information:
- Genomics, Vol. 34, Issue 3; Other Information: PBD: 15 Jun 1996
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
GENES
GENE REGULATION
DNA-CLONING
TRANSCRIPTION
DNA SEQUENCING
GENETIC MAPPING
TISSUE DISTRIBUTION
CHROMATIN
BIOSYNTHESIS
STRUCTURE-ACTIVITY RELATIONSHIPS
HUMAN CHROMOSOME 17
HUMAN CHROMOSOMES
NUCLEOTIDES
MICE
AMINO ACID SEQUENCE
SACCHAROMYCES CEREVISIAE
POLYMERASE CHAIN REACTION