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Title: Aptamers and methods for their in vitro selection and uses thereof

Patent ·
OSTI ID:983907
 [1];  [2]
  1. Walnut Creek, CA
  2. Severna Park, MD
+ Show Author Affiliations

The present method is an improved in vitro selection protocol that relies on magnetic separations for DNA aptamer production that is relatively easy and scalable without the need for expensive robotics. The ability of aptamers selected by this method to recognize and bind their target protein with high affinity and specificity, and detail their uses in a number of assays is also described. Specific TTF1 and His6 aptamers were selected using the method described, and shown to be useful for enzyme-linked assays, Western blots, and affinity purification.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC03-76SF00098
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
7,329,742
Application Number:
s 10/934,856
OSTI ID:
983907
Country of Publication:
United States
Language:
English

References (9)

Selection of RNA Aptamers to the Alzheimer's Disease Amyloid Peptide journal February 2002
Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase journal August 1990
Aptamer affinity chromatography: journal August 1999
An improved method for the in vitro evolution of aptamers and applications in protein detection and purification journal September 2003
Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures journal February 1992
Automated Acquisition of Aptamer Sequences journal June 2002
In vitro selection of RNA molecules that bind specific ligands journal August 1990
Automated selection of aptamers against protein targets translated in vitro: from gene to aptamer journal October 2002
High-throughput protein arrays: prospects for molecular diagnostics journal June 2002

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES