Regulating expression of cell and tissue-specific genes by modifying transcription

Beachy, Roger N; Dai, Shunhong

doi:10.2172/983703

Title: Regulating expression of cell and tissue-specific genes by modifying transcription

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Abstract

Transcriptional regulation is the primary step to control gene expression, therefore function. Such regulation is achieved primarily via a combination of the activities of the promoter cis regulatory DNA elements and trans regulatory proteins that function through binding to these DNA elements. Rice bZIP transcription factors RF2a, RF2b and RLP1 play key roles in regulating the activity of a vascular tissue specific promoter isolated from Rice Tungro Bacilliform Virus (RTBV), through their interactions with the Box II essential cis element located in the promoter (Dai et al., 2006., Dai et al., 2004., Yin et al., 1997). RF2a, RF2b and RLP1 possess multiple regulatory domains. Functional characterization reveals that those domains can activate or repress the activity of the RTBV promoter. It is equally as important to recognize that these proteins control plant development by regulating differentiation and/or function of the vascular tissues. Studies of transcriptional regulation of the RTBV promoter by this group of bZIP proteins will not only provide insights about gene expression in the vascular tissue, but also insights about general mechanisms of transcription activation and repression. The knowledge gained from this research will also enable us to develop a well-described set of tools that can be usedmore »« less

Authors:: Beachy, Roger N; Dai, Shunhong

Publication Date:: Mon Jun 14 00:00:00 EDT 2010

Research Org.:: Donald Danforth Plant Science Center, St. Louis, MO

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 983703

Report Number(s):: DOE-ER20355
10SC000997; CFDA 81.049

DOE Contract Number:: FG02-99ER20355

Resource Type:: Technical Report

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; transcription, rice, promoter

Citation Formats


                    Beachy, Roger N, and Dai, Shunhong. Regulating expression of cell and tissue-specific genes by modifying transcription.  United States: N. p., 2010. 
        Web.  doi:10.2172/983703.

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                    Beachy, Roger N, & Dai, Shunhong. Regulating expression of cell and tissue-specific genes by modifying transcription.  United States.  https://doi.org/10.2172/983703

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                    Beachy, Roger N, and Dai, Shunhong. 2010.  
        "Regulating expression of cell and tissue-specific genes by modifying transcription".  United States.  https://doi.org/10.2172/983703.  https://www.osti.gov/servlets/purl/983703.

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@article{osti_983703,

  title        = {Regulating expression of cell and tissue-specific genes by modifying transcription},

  author       = {Beachy, Roger N and Dai, Shunhong},

  abstractNote = {Transcriptional regulation is the primary step to control gene expression, therefore function. Such regulation is achieved primarily via a combination of the activities of the promoter cis regulatory DNA elements and trans regulatory proteins that function through binding to these DNA elements. Rice bZIP transcription factors RF2a, RF2b and RLP1 play key roles in regulating the activity of a vascular tissue specific promoter isolated from Rice Tungro Bacilliform Virus (RTBV), through their interactions with the Box II essential cis element located in the promoter (Dai et al., 2006., Dai et al., 2004., Yin et al., 1997). RF2a, RF2b and RLP1 possess multiple regulatory domains. Functional characterization reveals that those domains can activate or repress the activity of the RTBV promoter. It is equally as important to recognize that these proteins control plant development by regulating differentiation and/or function of the vascular tissues. Studies of transcriptional regulation of the RTBV promoter by this group of bZIP proteins will not only provide insights about gene expression in the vascular tissue, but also insights about general mechanisms of transcription activation and repression. The knowledge gained from this research will also enable us to develop a well-described set of tools that can be used to control expression of multiple genes in transgenic plants. We have proposed characterize the function domains of RF2a, RF2b and RLP1 and explore the biological function of the transcription repressor RLP1.},

  doi          = {10.2172/983703},

  url          = {https://www.osti.gov/biblio/983703},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Mon Jun 14 00:00:00 EDT 2010},

  month        = {Mon Jun 14 00:00:00 EDT 2010}

}

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