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Title: Cellobiohydrolase I gene and improved variants

Patent ·
OSTI ID:982999
 [1];  [2];  [3];  [1];  [4];  [5];  [1]
  1. Golden, CO
  2. Berthoud, CO
  3. San Carlos, CA
  4. Lakewood, CO
  5. Littleton, CO
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The disclosure provides a method for preparing an active exoglucanase in a heterologous host of eukaryotic origin. The method includes mutagenesis to reduce glycosylation of the exoglucanase when expressed in a heterologous host. It is further disclosed a method to produce variant cellobiohydrolase that is stable at high temperature through mutagenesis.

Research Organization:
National Energy Technology Laboratory (NETL), Pittsburgh, PA, Morgantown, WV (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-99G0-10337
Assignee:
MidWest Research Institute (Kansas City, MO)
Patent Number(s):
7,375,197
Application Number:
10/031,496
OSTI ID:
982999
Country of Publication:
United States
Language:
English

References (4)

Cloning and Expression of Trichoderma reesei Cellobiohydrolase I in Pichia pastoris journal October 1999
Selective elongation of the oligosaccharide attached to the second potential glycosylation site of yeast exoglucanase: effects on the activity and properties of the enzyme journal December 1994
Modified glycosylation of cellobiohydrolase I from a high cellulase-producing mutant strain of Trichoderma reesei journal August 1998
In vitro Conversion of the Carbohydrate Moiety of Fungal Glycoproteins to Mammalian-type Oligosaccharides. Evidence for N -acetylglucosaminyltransferase-I-accepting Glycans from Trichoderma reesei journal November 1997

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