Siderophore production and facilitated uptake of iron plutonium in p. putida.
- Hakim
- Joseph G.
- Sean D.
- Larry E.
- Mary P.
Bioremediation is a very attractive alternative for restoration of contaminated soil and ground water . This is particularly true for radionuclide contamination, which tends to be low in concentration and distributed over large surface areas . Microorganisms, through their natural metabolism, produce a large variety of organic molecules of different size and functionality . These molecules interact with contaminants present in the microbe's environment . Through these interactions bio-molecules can solubilize, oxidize, reduce or precipitate major metal contaminant in soils and ground water . We are studying these interaction for actinides and common soil subsurface bacteria . One focus has been on siderophores, small molecules that have great affinity for hard metal ions, and their potential to affect the distribution and mobility of actinide contaminants . The metal siderophores assembly can be recognized and taken up by micro-organisms through their interference with their iron uptake system . The first step in the active iron transport consists of Fe(III)-siderophore recognition by membrane receptors, which requires specific stereo orientation of the Fe(III)-siderophore complex . Recent investigations have shown that siderophores can form strong complexes with a large variety of toxic metals and may mediate their introduction inside the cell . We have previously shown that a Puhydroxamate siderophore assembly is recognized and taken up by the Microbacterium flavescens (JG-9). However, it is not clear if Pu-siderophore assemblies of other siderophores are also recognized.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 976553
- Report Number(s):
- LA-UR-03-0913; TRN: US201017%%700
- Resource Relation:
- Journal Volume: 673; Conference: Submitted to: Plutonium Futures Conference, July 6-10, Albuquerque, NM
- Country of Publication:
- United States
- Language:
- English
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