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Title: Synthesis of DNA

Patent ·
OSTI ID:957000

A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence. Preselected sequence segments that will complete the desired double-stranded DNA are determined. Preselected segment sequences of DNA that will be used to complete the desired double-stranded DNA are provided. The preselected segment sequences of DNA are assembled to produce the desired double-stranded DNA.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
W-7405-ENG-48
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Number(s):
7,452,666
Application Number:
10/394,911
OSTI ID:
957000
Country of Publication:
United States
Language:
English

References (1)

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides journal October 1995