Overcoming the anaerobic hurdle in phenotypic microarrays: Generation andvisualization of growth curve data for Desulfovibrio vulgaris Hildenborough
Growing anaerobic microorganisms in phenotypic microarrays (PM) and 96-well microtiter plates is an emerging technology that allows high throughput survey of the growth and physiology and/or phenotype of cultivable microorganisms. For non-model bacteria, a method for phenotypic analysis is invaluable, not only to serve as a starting point for further evaluation, but also to provide a broad understanding of the physiology of an uncharacterized wild-type organism or the physiology/phenotype of a newly created mutant of that organism. Given recent advances in genetic characterization and targeted mutations to elucidate genetic networks and metabolic pathways, high-throughput methods for determining phenotypic differences are essential. Here we outline challenges presented in studying the physiology and phenotype of a sulfate reducing anaerobic delta proteobacterium, Desulfovibrio vulgaris Hildenborough. Modifications of the commercially available OmniLog(TM) system (Hayward, CA) for experimental setup, and configuration, as well as considerations in PM data analysis are presented. Also highlighted here is data viewing software that enables users to view and compare multiple PM data sets. The PM method promises to be a valuable strategy in our systems biology approach to D. vulgaris studies and is readily applicable to other anaerobic and aerobic bacteria.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- Earth Sciences Division
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 953769
- Report Number(s):
- LBNL-1822E; JMIMDQ; TRN: US201002%%891
- Journal Information:
- Journal of Microbiological Methods, Vol. 76; ISSN 0167-7012
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
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BACTERIA
BIOLOGICAL PATHWAYS
BIOLOGY
CONFIGURATION
DATA ANALYSIS
DESULFOVIBRIO
EVALUATION
GENETICS
MICROORGANISMS
MODIFICATIONS
MUTANTS
MUTATIONS
PHENOTYPE
PHYSIOLOGY
PLATES
SULFATES
Desulfovibrio vulgaris Hildenborough
Sulfate reducing bacteria
Phenotypic microarray
Omnilog
Anaerobes
Phenotype
pH stress
NaCl stress