Is Increased Low-dose somatic Radiosensitivity Associated with Increased Transgenerational Germline Mutation
Using single-molecule polymerase chain reaction, the frequency of spontaneous and radiation-induced mutation at an expanded simple tandem repeat (ESTR) locus was studied in DNA samples extracted from sperm and bone marrow of Atm knockout (Atm+/–) heterozygous male mice. The frequency of spontaneous mutation in sperm and bone marrow in Atm+/– males did not significantly differ from that in wild-type BALB/c mice. Acute gamma-ray exposure did not affect ESTR mutation frequency in bone marrow and resulted in similar increases in sperm samples taken from Atm+/– and BALB/c males. Taken together, these results suggest that the Atm haploinsufficiency analyzed in our study does not affect spontaneous and radiation-induced ESTR mutation frequency in mice.
- Research Organization:
- Columbia University Medical Center, Center for Radiological Research
- Sponsoring Organization:
- USDOE Office of Science (SC)
- DOE Contract Number:
- FG02-03ER63631
- OSTI ID:
- 938982
- Report Number(s):
- DOE/ER/63631; TRN: US0902919
- Country of Publication:
- United States
- Language:
- English
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