Extraction of PCR-amplifiable genomic DNA from Bacillus anthracisspores
Bacterial endospore disruption and nucleic acid extractionresulting in DNA of PCR-amplifiable quality and quantity are not trivial.Responding to the needs of the Hazardous Materials Response Unit (HMRU),Laboratory Division, Federal Bureau of Investigation, protocols weredeveloped to close these gaps. Effectiveness and reproducibility of thetechniques were validated with laboratory grown pure spores of Bacillusanthracis and its close phylogenetic neighbors, and with spiked soils anddamaged samples.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE; FBI. S8I863013D
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 917812
- Report Number(s):
- LBNL-52707; R&D Project: 43AP01; TRN: US200817%%885
- Country of Publication:
- United States
- Language:
- English
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