Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby

Waldo, Geoffrey S

Title: Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby

Patent · Tue Sep 18 00:00:00 EDT 2007

OSTI ID:915234

Waldo, Geoffrey S ^[1]

Santa Fe, NM

The current invention provides methods of improving folding of polypeptides using a poorly folding domain as a component of a fusion protein comprising the poorly folding domain and a polypeptide of interest to be improved. The invention also provides novel green fluorescent proteins (GFPs) and red fluorescent proteins that have enhanced folding properties.

View Patent

Cite

Export

Save

Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: W-7405-ENG-36

Assignee:: Los Alamos National Security, LLC (Los Alamos, NM)

Patent Number(s):: 7,271,241

Application Number:: 10/423,688

OSTI ID:: 915234

Country of Publication:: United States

Language:: English

References (24)

Dynamic and quantitative Ca2+ measurements using improved cameleons Miyawaki, A.; Griesbeck, O.; Heim, R. Proceedings of the National Academy of Sciences, Vol. 96, Issue 5 https://doi.org/10.1073/pnas.96.5.2135	journal	March 1999
Natural Animal Coloration Can Be Determined by a Nonfluorescent Green Fluorescent Protein Homolog Lukyanov, Konstantin A.; Fradkov, Arkady F.; Gurskaya, Nadya G. Journal of Biological Chemistry, Vol. 275, Issue 34 https://doi.org/10.1074/jbc.C000338200	journal	June 2000
Comparison of biosequences Smith, Temple F.; Waterman, Michael S. Advances in Applied Mathematics, Vol. 2, Issue 4, p. 482-489 https://doi.org/10.1016/0196-8858(81)90046-4	journal	December 1981
In-vitro Selection of Highly Stabilized Protein Variants with Optimized Surface Martin, Andreas; Sieber, Volker; Schmid, Franz X. Journal of Molecular Biology, Vol. 309, Issue 3, p. 717-726 https://doi.org/10.1006/jmbi.2001.4698	journal	June 2001
Major colour patterns of reef-building corals are due to a family of GFP-like proteins Dove, S. G.; Hoegh-Guldberg, O.; Ranganathan, S. Coral Reefs, Vol. 19, Issue 3 https://doi.org/10.1007/PL00006956	journal	January 2001
Fluorescent proteins from nonbioluminescent Anthozoa species Matz, Mikhail V.; Fradkov, Arkady F.; Labas, Yulii A. Nature Biotechnology, Vol. 17, Issue 10 https://doi.org/10.1038/13657	journal	October 1999
Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed) Bevis, Brooke J.; Glick, Benjamin S. Nature Biotechnology, Vol. 20, Issue 1 https://doi.org/10.1038/nbt0102-83	journal	January 2002
Rapid evolution of a protein in vitro by DNA shuffling Stemmer, Willem P. C. Nature, Vol. 370, Issue 6488 https://doi.org/10.1038/370389a0	journal	August 1994
Rapid protein-folding assay using green fluorescent protein Waldo, Geoffrey S.; Standish, Blake M.; Berendzen, Joel Nature Biotechnology, Vol. 17, Issue 7, p. 691-695 https://doi.org/10.1038/10904	journal	July 1999
"Fluorescent Timer": Protein That Changes Color with Time Terskikh, A. Science, Vol. 290, Issue 5496 https://doi.org/10.1126/science.290.5496.1585	journal	November 2000
Display of Expression Products of cDNA Libraries on Phage Surfaces. A Versatile Screening System for Selective Isolation of Genes by Specific Gene-Product/Ligand Interaction Crameri, Reto; Jaussi, Rolf; Menz, Gunter European Journal of Biochemistry, Vol. 226, Issue 1 https://doi.org/10.1111/j.1432-1033.1994.tb20025.x	journal	November 1994
Creating new fluorescent probes for cell biology Zhang, Jin; Campbell, Robert E.; Ting, Alice Y. Nature Reviews Molecular Cell Biology, Vol. 3, Issue 12 https://doi.org/10.1038/nrm976	journal	December 2002
The Green Fluorescent Protein Tsien, Roger Y. Annual Review of Biochemistry, Vol. 67, Issue 1, p. 509-544 https://doi.org/10.1146/annurev.biochem.67.1.509	journal	June 1998
The production, purification and crystallization of a pocilloporin pigment from a reef-forming coral Beddoe, Travis; Ling, Michael; Dove, Sophie Acta Crystallographica Section D Biological Crystallography, Vol. 59, Issue 3, p. 597-599 https://doi.org/10.1107/S0907444902023466	journal	February 2003
GFP-like chromoproteins as a source of far-red fluorescent proteins Gurskaya, Nadya G.; Fradkov, Arkady F.; Terskikh, Alexey FEBS Letters, Vol. 507, Issue 1, p. 16-20 https://doi.org/10.1016/S0014-5793(01)02930-1	journal	September 2001
DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution. Stemmer, W. P. Proceedings of the National Academy of Sciences, Vol. 91, Issue 22 https://doi.org/10.1073/pnas.91.22.10747	journal	October 1994
Redesigning Enzyme Topology by Directed Evolution MacBeath, G. Science, Vol. 279, Issue 5358, p. 1958-1961 https://doi.org/10.1126/science.279.5358.1958	journal	March 1998
Crystal Structure of the Aequorea victoria Green Fluorescent Protein Orm , M.; Cubitt, A. B.; Kallio, K. Science, Vol. 273, Issue 5280 https://doi.org/10.1126/science.273.5280.1392	journal	September 1996
Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling Crameri, Andreas; Whitehorn, Erik A.; Tate, Emily Nature Biotechnology, Vol. 14, Issue 3 https://doi.org/10.1038/nbt0396-315	journal	March 1996
Directed evolution: Creating biocatalysts for the future Arnold, Frances H. Chemical Engineering Science, Vol. 51, Issue 23 https://doi.org/10.1016/S0009-2509(96)00288-6	journal	December 1996
The molecular structure of green fluorescent protein Yang, Fan; Moss, Larry G.; Phillips, George N. Nature Biotechnology, Vol. 14, Issue 10 https://doi.org/10.1038/nbt1096-1246	journal	October 1996
Searching Sequence Space Stemmer, Willem P. C. Nature Biotechnology, Vol. 13, Issue 6 https://doi.org/10.1038/nbt0695-549	journal	June 1995
A naturally enhanced green fluorescent protein from magnificent sea anemone (Heteractis magnifica) and its functional analysis Tu, Hongbin; Xiong, Qian; Zhen, Suilan Biochemical and Biophysical Research Communications, Vol. 301, Issue 4, p. 879-885 https://doi.org/10.1016/S0006-291X(03)00019-6	journal	February 2003
Green Fluorescent Protein (GFP): Applications, Structure, and Related Photophysical Behavior Zimmer, Marc Chemical Reviews, Vol. 102, Issue 3 https://doi.org/10.1021/cr010142r	journal	March 2002

Similar Records

Engineering and Characterization of a Superfolder Green Fluorescent Protein

Journal Article · Sun Jan 01 00:00:00 EST 2006 · Nat. Biotechnol. · OSTI ID:915234

Pedelacq, J; Cabantous, S; Tran, T; +2 more

Protein- protein interaction detection system using fluorescent protein microdomains

Patent · Tue Feb 23 00:00:00 EST 2010 · OSTI ID:915234

Waldo, Geoffrey S; Cabantous, Stephanie

New Molecular Reporters for Rapid Protein Folding Assays

Journal Article · Wed Jun 11 00:00:00 EDT 2008 · PLoS ONE · OSTI ID:915234

Cabantous, Stéphanie; Rogers, Yvonne; Terwilliger, Thomas C.; +1 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES

Title: Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby

Citation Formats

References (24)

Similar Records

Related Subjects