The Effect of Coenzyme A on the Metabolic Oxidation of LabeledFatty Acids: Rate Studies, Instrumentation, and Liver Fractionation
The effect of pantothenic acid deficiency on the rate of C{sup 14}O{sub 2} excretion and on distribution of radioactivity in liver fractions has been studied in rats given sodium acetate-2-C{sup 14} and sodium heptanoate-7-C{sup 14} The rate of excretion of breath C{sub 14}O has been measured by use of a method in which a sensitive ionization chamber and electrometer directly and continuously record carbon-14 excretion. The labeled fatty acids are more rapidly metabolized to C{sup 14}O{sub 2} in PAD rats than in normal rats. CoA depresses the C{sup 14}O excretion 2 in both normal and PAD rats in experiments with either labeled acid. There are differences in the oxidation of these two fatty acids, and the differences are consistent with postulated metabolic schemes. CoA increases radioactivity deposited in the fat of the liver, but does not appreciably change the radioactivity incorporated in the protein and nonsaponifiable lipid fractions.
- Research Organization:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USAEC
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 914457
- Report Number(s):
- UCRL-2849; TRN: US200809%%74
- Country of Publication:
- United States
- Language:
- English
Similar Records
The effect of alterations in total coenzyme A on metabolic pathways in the liver and heart
Mangiferin treatment inhibits hepatic expression of acyl-coenzyme A:diacylglycerol acyltransferase-2 in fructose-fed spontaneously hypertensive rats: a link to amelioration of fatty liver