Characterizing the Catalytic Potential of Deinococcus, Arthrobacter and other Robust Bacteria in Contaminated Subsurface Environments of the Hanford Site

Fredrickson, Jim K; Daly, Michael J

doi:10.2172/895911

Title: Characterizing the Catalytic Potential of Deinococcus, Arthrobacter and other Robust Bacteria in Contaminated Subsurface Environments of the Hanford Site

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Abstract

Until recently, there have been no clear physiologic predictors of a cell's ability to recover from ionizing radiation (IR), desiccation, and other DOE-relevant oxidative stress conditions. In general, the most resistant bacteria have been Gram-positive (e.g., Deinococcus, Arthrobacter, Lactobacillus & Enterococcus spp.) and the most sensitive have been Gram-negative (e.g., Pseudomonas, Shewanella & Neisseria spp.). However, there are several reported exceptions to this paradigm, the Gram-negative cyanobacterium Chroococcidiopsis is extremely resistant to IR, whereas the Gram-positive Micrococcus luteus is sensitive. We have identified biomolecular signatures for radiation sensitivity and resistance which are independent of phylogeny, where very high and very low intracellular Mn/Fe concentration ratios correlated with very high and very low resistances, respectively; and restricting Mn(II) in the famously resistant Deinococcus radiodurans sensitized this eubacterium to IR (http://cfyn.ifas.ufl.edu/radiation.pdf).

Authors:: Fredrickson, Jim K; Daly, Michael J

Publication Date:: Thu Jun 01 00:00:00 EDT 2006

Research Org.:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Henry M. Jackson Foundation, Uniformed Services University of the Health Sciences

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 895911

Report Number(s):: ERSD-1024843-2006
R&D Project: ERSD 1024843; TRN: US0700554

Resource Type:: Technical Report

Country of Publication:: United States

Language:: English

Subject:: 12 MANAGEMENT OF RADIOACTIVE WASTES, AND NON-RADIOACTIVE WASTES FROM NUCLEAR FACILITIES; 54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; BACTERIA; CONCENTRATION RATIO; IONIZING RADIATIONS; LACTOBACILLUS; MICROCOCCUS LUTEUS; PSEUDOMONAS; RADIATIONS; SENSITIVITY

Citation Formats


                    Fredrickson, Jim K, and Daly, Michael J. Characterizing the Catalytic Potential of Deinococcus, Arthrobacter and other Robust Bacteria in Contaminated Subsurface Environments of the Hanford Site.  United States: N. p., 2006. 
        Web.  doi:10.2172/895911.

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                    Fredrickson, Jim K, & Daly, Michael J. Characterizing the Catalytic Potential of Deinococcus, Arthrobacter and other Robust Bacteria in Contaminated Subsurface Environments of the Hanford Site.  United States.  https://doi.org/10.2172/895911

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                    Fredrickson, Jim K, and Daly, Michael J. 2006.  
        "Characterizing the Catalytic Potential of Deinococcus, Arthrobacter and other Robust Bacteria in Contaminated Subsurface Environments of the Hanford Site".  United States.  https://doi.org/10.2172/895911.  https://www.osti.gov/servlets/purl/895911.

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@article{osti_895911,

  title        = {Characterizing the Catalytic Potential of Deinococcus, Arthrobacter and other Robust Bacteria in Contaminated Subsurface Environments of the Hanford Site},

  author       = {Fredrickson, Jim K and Daly, Michael J},

  abstractNote = {Until recently, there have been no clear physiologic predictors of a cell's ability to recover from ionizing radiation (IR), desiccation, and other DOE-relevant oxidative stress conditions. In general, the most resistant bacteria have been Gram-positive (e.g., Deinococcus, Arthrobacter, Lactobacillus & Enterococcus spp.) and the most sensitive have been Gram-negative (e.g., Pseudomonas, Shewanella & Neisseria spp.). However, there are several reported exceptions to this paradigm, the Gram-negative cyanobacterium Chroococcidiopsis is extremely resistant to IR, whereas the Gram-positive Micrococcus luteus is sensitive. We have identified biomolecular signatures for radiation sensitivity and resistance which are independent of phylogeny, where very high and very low intracellular Mn/Fe concentration ratios correlated with very high and very low resistances, respectively; and restricting Mn(II) in the famously resistant Deinococcus radiodurans sensitized this eubacterium to IR (http://cfyn.ifas.ufl.edu/radiation.pdf).},

  doi          = {10.2172/895911},

  url          = {https://www.osti.gov/biblio/895911},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Thu Jun 01 00:00:00 EDT 2006},

  month        = {Thu Jun 01 00:00:00 EDT 2006}

}

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