Method To Identify Specific Inhibiutors Of Imp Dehydrogenase

Patent · Tue Nov 28 00:00:00 EST 2000

OSTI ID:879487

Collart, Frank R ^[1]; Huberman, Eliezer ^[2]

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1. Bolingbrook, IL
2. LaGrange, IL

This invention relates to methods to identify specific inhibitors of the purine nucleotide synthesis enzyme, IMP dehydrogenase (IMPDH). IMPDH is an essential enzyme found in all free-living organisms from humans to bacteria and is an important therapeutic target. The invention allows the identification of specific inhibitors of any IMPDH enzyme which can be expressed in a functional form in a recombinant host cell. A variety of eukaryotic or prokaryotic host systems commonly used for the expression of recombinant proteins are suitable for the practice of the invention. The methods are amenable to high throughput systems for the screening of inhibitors generated by combinatorial chemistry or other methods such as antisense molecule production. Utilization of exogenous guanosine as a control component of the methods allows for the identification of inhibitors specific for IMPDH rather than other causes of decreased cell proliferation.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL (United States)
• DOE Contract Number: W-31-109-ENG-38
• Assignee: The University of Chicago (Chicago, IL)
• Patent Number(s): US 6,153,398
• Application Number: 08/997758
• OSTI ID: 879487
• Country of Publication: United States
• Language: English