Mutant E. coli strain with increased succinic acid production

Patent · Tue Jan 01 00:00:00 EST 2002

OSTI ID:875302

Donnelly, Mark ^[1]; Millard, Cynthia S ^[2]; Stols, Lucy ^[3]

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1. Warrenville, IL
2. Plainfield, IL
3. Woodridge, IL

A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL (United States)
• DOE Contract Number: W-31109-ENG-38
• Assignee: University of Chicago (Chicago, IL)
• Patent Number(s): US RE37393
• OSTI ID: 875302
• Country of Publication: United States
• Language: English

Related Subjects

mutant
coli
strain
increased
succinic
acid
production
method
isolating
producing
bacteria
provided
comprising
increasing
biomass
organism
lacks
ability
catabolize
pyruvate
subjecting
glucose-rich
medium
anaerobic
environment
enable
pyruvate-catabolizing
mutants
grow
provides
produces
amounts
derived
parent
lacked
genes
formate
lyase
lactate
dehydrogenase
belongs
acid production
succinic acid
provided comprising
acid producing
producing bacteria
catabolize pyruvate
comprising increasing
coli strain
increased succinic
isolating succinic
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