Triplex in-situ hybridization
Abstract
Disclosed are methods for detecting in situ the presence of a target sequence in a substantially double-stranded nucleic acid segment, which comprises: a) contacting in situ under conditions suitable for hybridization a substantially double-stranded nucleic acid segment with a detectable third strand, said third strand being capable of hybridizing to at least a portion of the target sequence to form a triple-stranded structure, if said target sequence is present; and b) detecting whether hybridization between the third strand and the target sequence has occurred.
- Inventors:
- Publication Date:
- Research Org.:
- Princeton Univ., NJ (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 874795
- Patent Number(s):
- 6,461,810
- Application Number:
- 09/531,000
- Assignee:
- Princeton University (Princeton, NJ)
- DOE Contract Number:
- FG02-96ER62202
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2000 Sep 08
- Country of Publication:
- United States
- Language:
- English
- Subject:
- /435/999/
Citation Formats
Fresco, Jacques R., and Johnson, Marion D. Triplex in-situ hybridization. United States: N. p., 2002.
Web.
Fresco, Jacques R., & Johnson, Marion D. Triplex in-situ hybridization. United States.
Fresco, Jacques R., and Johnson, Marion D. 2002.
"Triplex in-situ hybridization". United States. https://www.osti.gov/servlets/purl/874795.
@article{osti_874795,
title = {Triplex in-situ hybridization},
author = {Fresco, Jacques R. and Johnson, Marion D.},
abstractNote = {Disclosed are methods for detecting in situ the presence of a target sequence in a substantially double-stranded nucleic acid segment, which comprises: a) contacting in situ under conditions suitable for hybridization a substantially double-stranded nucleic acid segment with a detectable third strand, said third strand being capable of hybridizing to at least a portion of the target sequence to form a triple-stranded structure, if said target sequence is present; and b) detecting whether hybridization between the third strand and the target sequence has occurred.},
doi = {},
url = {https://www.osti.gov/biblio/874795},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2002},
month = {Tue Jan 01 00:00:00 EST 2002}
}
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