Expression of multiple proteins in transgenic plants
Abstract
A method is disclosed for the production of multiple proteins in transgenic plants. A DNA construct for introduction into plants includes a provision to express a fusion protein of two proteins of interest joined by a linking domain including plant ubiquitin. When the fusion protein is produced in the cells of a transgenic plant transformed with the DNA construction, native enzymes present in plant cells cleave the fusion protein to release both proteins of interest into the cells of the transgenic plant. Since the proteins are produced from the same fusion protein, the initial quantities of the proteins in the cells of the plant are approximately equal.
- Inventors:
-
- Madison, WI
- Publication Date:
- Research Org.:
- The Consortium for Plant Biotechnology Research, Inc., West Lafayette, IN (US)
- OSTI Identifier:
- 874759
- Patent Number(s):
- US 6455759
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- DOE Contract Number:
- FC05-92OR22072
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- expression; multiple; proteins; transgenic; plants; method; disclosed; production; dna; construct; introduction; provision; express; fusion; protein; joined; linking; domain; including; plant; ubiquitin; produced; cells; transformed; construction; native; enzymes; cleave; release; initial; quantities; approximately; equal; plant cell; dna construct; fusion protein; transgenic plant; /800/
Citation Formats
Vierstra, Richard D, and Walker, Joseph M. Expression of multiple proteins in transgenic plants. United States: N. p., 2002.
Web.
Vierstra, Richard D, & Walker, Joseph M. Expression of multiple proteins in transgenic plants. United States.
Vierstra, Richard D, and Walker, Joseph M. 2002.
"Expression of multiple proteins in transgenic plants". United States. https://www.osti.gov/servlets/purl/874759.
@article{osti_874759,
title = {Expression of multiple proteins in transgenic plants},
author = {Vierstra, Richard D and Walker, Joseph M},
abstractNote = {A method is disclosed for the production of multiple proteins in transgenic plants. A DNA construct for introduction into plants includes a provision to express a fusion protein of two proteins of interest joined by a linking domain including plant ubiquitin. When the fusion protein is produced in the cells of a transgenic plant transformed with the DNA construction, native enzymes present in plant cells cleave the fusion protein to release both proteins of interest into the cells of the transgenic plant. Since the proteins are produced from the same fusion protein, the initial quantities of the proteins in the cells of the plant are approximately equal.},
doi = {},
url = {https://www.osti.gov/biblio/874759},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2002},
month = {Tue Jan 01 00:00:00 EST 2002}
}
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Works referenced in this record:
Novel applications of the ubiquitin-dependent proteolytic pathway in plant genetic engineering
journal, April 1992
- Hondred, David; Vierstra, Richard D.
- Current Opinion in Biotechnology, Vol. 3, Issue 2
Use of Ubiquitin Fusions to Augment Protein Expression in Transgenic Plants
journal, February 1999
- Hondred, David; Walker, Joseph M.; Mathews, Dennis E.
- Plant Physiology, Vol. 119, Issue 2
Cloning and characterization of a 20-kDa ubiquitin carrier protein from wheat that catalyzes multiubiquitin chain formation in vitro.
journal, November 1991
- Van Nocker, S.; Vierstra, R. D.
- Proceedings of the National Academy of Sciences, Vol. 88, Issue 22