Methods and compositions for efficient nucleic acid sequencing

Patent · Tue Jan 01 00:00:00 EST 2002

OSTI ID:874493

Drmanac, Radoje ^[1]

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1. 850 E. Greenwich Pl., Palo Alto, CA 94303

Disclosed are novel methods and compositions for rapid and highly efficient nucleic acid sequencing based upon hybridization with two sets of small oligonucleotide probes of known sequences. Extremely large nucleic acid molecules, including chromosomes and non-amplified RNA, may be sequenced without prior cloning or subcloning steps. The methods of the invention also solve various current problems associated with sequencing technology such as, for example, high noise to signal ratios and difficult discrimination, attaching many nucleic acid fragments to a surface, preparing many, longer or more complex probes and labelling more species.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL (United States)
• DOE Contract Number: W-31109-ENG-38
• Assignee: Drmanac; Radoje (850 E. Greenwich Pl., Palo Alto, CA 94303)
• Patent Number(s): US 6401267
• OSTI ID: 874493
• Country of Publication: United States
• Language: English

Related Subjects

methods
compositions
efficient
nucleic
acid
sequencing
disclosed
novel
rapid
highly
based
hybridization
sets
oligonucleotide
probes
sequences
extremely
molecules
including
chromosomes
non-amplified
rna
sequenced
prior
cloning
subcloning
steps
solve
current
associated
technology
example
noise
signal
ratios
difficult
discrimination
attaching
fragments
surface
preparing
complex
labelling
species
nucleic acid
highly efficient
acid sequencing
oligonucleotide probe
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