Method of detecting genetic translocations identified with chromosomal abnormalities

Gray, Joe W; Pinkel, Daniel; Tkachuk, Douglas

Title: Method of detecting genetic translocations identified with chromosomal abnormalities

Patent · Mon Jan 01 00:00:00 EST 2001

OSTI ID:873952

Gray, Joe W ^[1]; Pinkel, Daniel ^[2]; Tkachuk, Douglas ^[1]

Livermore, CA
Walnut Creek, CA

Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML) and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

DOE Contract Number:: W-7405-ENG-48

Assignee:: Regents of University of California (Oakland, CA)

Patent Number(s):: US 6280929

OSTI ID:: 873952

Country of Publication:: United States

Language:: English

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genetic
translocations
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chromosomal
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employ nucleic
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disease related
/435/436/999/

Title: Method of detecting genetic translocations identified with chromosomal abnormalities

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