Microfabricated capillary array electrophoresis device and method

Simpson, Peter C; Mathies, Richard A; Woolley, Adam T

Title: Microfabricated capillary array electrophoresis device and method

Patent · Sat Jan 01 00:00:00 EST 2000

OSTI ID:873356

Simpson, Peter C ^[1]; Mathies, Richard A ^[2]; Woolley, Adam T ^[3]

Oakland, CA
Moraga, CA
Belmont, MA

A capillary array electrophoresis (CAE) micro-plate with an array of separation channels connected to an array of sample reservoirs on the plate. The sample reservoirs are organized into one or more sample injectors. One or more waste reservoirs are provided to collect wastes from reservoirs in each of the sample injectors. Additionally, a cathode reservoir is also multiplexed with one or more separation channels. To complete the electrical path, an anode reservoir which is common to some or all separation channels is also provided on the micro-plate. Moreover, the channel layout keeps the distance from the anode to each of the cathodes approximately constant.

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Research Organization:: Univ. of California (United States)

DOE Contract Number:: FG03-91ER61125

Assignee:: Regents of University of California (Berkeley, CA)

Patent Number(s):: US 6143152

OSTI ID:: 873356

Country of Publication:: United States

Language:: English

References (32)

Fluorescence scanner employing a macro scanning objective Alexay, Christopher C.; Kain, Robert C.; Hanzel, David K. Photonics West '96, SPIE Proceedings https://doi.org/10.1117/12.236191	conference	March 1996
High-throughput genetic analysis using microfabricated 96-sample capillary array electrophoresis microplates Simpson, P. C.; Roach, D.; Woolley, A. T. Proceedings of the National Academy of Sciences, Vol. 95, Issue 5 https://doi.org/10.1073/pnas.95.5.2256	journal	March 1998
Microfabrication Technology for the Production of Capillary Array Electrophoresis Chips Simpson, Peter C.; Woolley, Adam T.; Mathies, Richard A. Biomedical Microdevices, Vol. 1, Issue 1, p. 7-26 https://doi.org/10.1023/A:1009922004301	journal	September 1998
Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillaries Ueno, Kyoji.; Yeung, Edward S. Analytical Chemistry, Vol. 66, Issue 9 https://doi.org/10.1021/ac00081a010	journal	April 1994
High-speed separations of DNA sequencing reactions by capillary electrophoresis Drossman, Howard.; Luckey, John A.; Kostichka, Anthony J. Analytical Chemistry, Vol. 62, Issue 9 https://doi.org/10.1021/ac00208a003	journal	May 1990
A novel MHC class I–like gene is mutated in patients with hereditary haemochromatosis Feder, J. N.; Gnirke, A.; Thomas, W. Nature Genetics, Vol. 13, Issue 4 https://doi.org/10.1038/ng0896-399	journal	August 1996
Energy-transfer fluorescent reagents for DNA analyses Glazer, Alexander N.; Mathies, Richard A. Current Opinion in Biotechnology, Vol. 8, Issue 1 https://doi.org/10.1016/S0958-1669(97)80163-2	journal	February 1997
High-performance electrophoresis Hjertén, Stellan Journal of Chromatography A, Vol. 347 https://doi.org/10.1016/S0021-9673(01)95485-8	journal	January 1985
Ultra-High-Speed DNA Sequencing Using Capillary Electrophoresis Chips Woolley, Adam T.; Mathies, Richard A. Analytical Chemistry, Vol. 67, Issue 20 https://doi.org/10.1021/ac00116a010	journal	October 1995
Functional Integration of PCR Amplification and Capillary Electrophoresis in a Microfabricated DNA Analysis Device Woolley, Adam T.; Hadley, Dean; Landre, Phoebe Analytical Chemistry, Vol. 68, Issue 23 https://doi.org/10.1021/ac960718q	journal	January 1996
Multiple Sheath-Flow Gel Capillary-Array Electrophoresis for Multicolor Fluorescent DNA Detection Takahashi, Satoshi.; Murakami, Katsuhiko.; Anazawa, Takashi. Analytical Chemistry, Vol. 66, Issue 7 https://doi.org/10.1021/ac00079a016	journal	April 1994
Capillary electrophoresis and sample injection systems integrated on a planar glass chip Harrison, D. Jed.; Manz, Andreas.; Fan, Zhonghui. Analytical Chemistry, Vol. 64, Issue 17 https://doi.org/10.1021/ac00041a030	journal	September 1992
Separation and analysis of DNA sequence reaction products by capillary gel electrophoresis Cohen, A. S.; Najarian, D. R.; Karger, B. L. Journal of Chromatography A, Vol. 516, Issue 1 https://doi.org/10.1016/S0021-9673(01)90203-1	journal	September 1990
Accessing Genetic Information with High-Density DNA Arrays Chee, M.; Yang, R.; Hubbell, E. Science, Vol. 274, Issue 5287 https://doi.org/10.1126/science.274.5287.610	journal	October 1996
Microsatellite-based cancer detection using capillary array electrophoresis and energy-transfer fluorescent primers Wang, Yiwen; Hung, Su-Chun; Linn, Jürgen F. Electrophoresis, Vol. 18, Issue 10 https://doi.org/10.1002/elps.1150181007	journal	January 1997
Glass chips for high-speed capillary electrophoresis separations with submicrometer plate heights Effenhauser, Carlo S.; Manz, Andreas.; Widmer, H. Michael. Analytical Chemistry, Vol. 65, Issue 19 https://doi.org/10.1021/ac00067a015	journal	October 1993
High-Speed Separation of Antisense Oligonucleotides on a Micromachined Capillary Electrophoresis Device Effenhauser, Carlo S.; Paulus, Aran.; Manz, Andreas. Analytical Chemistry, Vol. 66, Issue 18 https://doi.org/10.1021/ac00090a024	journal	September 1994
Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips. Woolley, A. T.; Mathies, R. A. Proceedings of the National Academy of Sciences, Vol. 91, Issue 24 https://doi.org/10.1073/pnas.91.24.11348	journal	November 1994
Transport, Manipulation, and Reaction of Biological Cells On-Chip Using Electrokinetic Effects Li, Paul C. H.; Harrison, D. Jed Analytical Chemistry, Vol. 69, Issue 8 https://doi.org/10.1021/ac9606564	journal	April 1997
DNA sequencing using capillary array electrophoresis Huang, Xiaohua C.; Quesada, Mark A.; Mathies, Richard A. Analytical Chemistry, Vol. 64, Issue 18 https://doi.org/10.1021/ac00042a021	journal	September 1992
A simple salting out procedure for extracting DNA from human nucleated cells Miller, S. A.; Dykes, D. D.; Polesky, H. F. Nucleic Acids Research, Vol. 16, Issue 3 https://doi.org/10.1093/nar/16.3.1215	journal	January 1988
High Speed Automated DNA Sequencing in Ultrathin Slab Gels Kostichka, Anthony J.; Marchbanks, Michael L.; Brumley, Robert L. Nature Biotechnology, Vol. 10, Issue 1 https://doi.org/10.1038/nbt0192-78	journal	January 1992
DNA sequencing using a four-color confocal fluorescence capillary array scanner Kheterpal, Indu; Scherer, James R.; Clark, Steven M. Electrophoresis, Vol. 17, Issue 12, p. 1852-1859 https://doi.org/10.1002/elps.1150171209	journal	January 1996
Capillary array electrophoresis using laser-excited confocal fluorescence detection Huang, Xiaohua C.; Quesada, Mark A.; Mathies, Richard A. Analytical Chemistry, Vol. 64, Issue 8 https://doi.org/10.1021/ac00032a025	journal	April 1992
Microchip Capillary Electrophoresis with an Integrated Postcolumn Reactor Jacobson, Stephen C.; Koutny, Lance B.; Hergenroeder, Roland. Analytical Chemistry, Vol. 66, Issue 20 https://doi.org/10.1021/ac00092a027	journal	October 1994
Integrated Microdevice for DNA Restriction Fragment Analysis Jacobson, Stephen C.; Ramsey, J. Michael Analytical Chemistry, Vol. 68, Issue 5 https://doi.org/10.1021/ac951230c	journal	January 1996
Multiplex dsDNA Fragment Sizing Using Dimeric Intercalation Dyes and Capillary Array Electrophoresis: Ionic Effects on the Stability and Electrophoretic Mobility of DNA−Dye Complexes Clark, Steven M.; Mathies, Richard A. Analytical Chemistry, Vol. 69, Issue 7 https://doi.org/10.1021/ac960866g	journal	April 1997
High-resolution capillary array electrophoretic sizing of multiplexed short tandem repeat loci using energy-transfer fluorescent primers Wang, Yiwen; Wallin, Jeanette M.; Ju, Jingyue Electrophoresis, Vol. 17, Issue 9 https://doi.org/10.1002/elps.1150170913	journal	January 1996
Capillary gel electrophoresis for rapid, high resolution DNA sequencing Swerdlow, Harold; Gesteland, Raymond Nucleic Acids Research, Vol. 18, Issue 6 https://doi.org/10.1093/nar/18.6.1415	journal	January 1990
High-Speed Parallel Separation of DNA Restriction Fragments Using Capillary Array Electrophoresis Clark, S. M.; Mathies, R. A. Analytical Biochemistry, Vol. 215, Issue 2 https://doi.org/10.1006/abio.1993.1571	journal	December 1993
Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip Harrison, D. J.; Fluri, K.; Seiler, K. Science, Vol. 261, Issue 5123 https://doi.org/10.1126/science.261.5123.895	journal	August 1993
Global prevalence of putative haemochromatosis mutations. Merryweather-Clarke, A. T.; Pointon, J. J.; Shearman, J. D. Journal of Medical Genetics, Vol. 34, Issue 4 https://doi.org/10.1136/jmg.34.4.275	journal	April 1997

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waste
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collect
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multiplexed
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layout
distance
cathodes
approximately
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channels connected
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electrical path
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Title: Microfabricated capillary array electrophoresis device and method

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References (32)

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