Methods for immobilizing nucleic acids on a gel substrate

Patent · Fri Jan 01 00:00:00 EST 1999

OSTI ID:872654

Mirzabekov, Andrei Darievich ^[1]; Proudnikov, Dimitri Y ^[2]; Timofeev, Edward N ^[1]; Kochetkova, Svetlana V ^[1]; Florentiev, Vladimir L ^[1]; Shick, Valentine V ^[1]

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1. Moscow, RU
2. Samara, RU

A method for labeling oligonucleotide molecules, and for immobilizing oligonucleotide and DNA molecules is provided comprising modifying the molecules to create a chemically active group, and contacting activated fluorescent dyes to the region. A method for preparing an immobilization substrate is also provided comprising modifying a gel to contain desired functional groups which covalently interact with certain moieties of the oligonucleotide molecules. A method for immobilizing biomolecules and other molecules within a gel by copolymerization of allyl-substituted oligonucleotides, DNA and proteins with acrylamide is also provided.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL (United States)
• DOE Contract Number: W-31109-ENG-38
• Assignee: University of Chicago (Chicago, IL)
• Patent Number(s): US 5981734
• OSTI ID: 872654
• Country of Publication: United States
• Language: English

Related Subjects

methods
immobilizing
nucleic
acids
substrate
method
labeling
oligonucleotide
molecules
dna
provided
comprising
modifying
create
chemically
active
contacting
activated
fluorescent
dyes
region
preparing
immobilization
contain
desired
functional
covalently
interact
moieties
biomolecules
copolymerization
allyl-substituted
oligonucleotides
proteins
acrylamide
nucleic acid
provided comprising
nucleic acids
oligonucleotide molecules
fluorescent dyes
dna molecules
dna molecule
fluorescent dye
chemically active
immobilizing biomolecules
/999/428/