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Title: Time-resolved fluorescence decay measurements for flowing particles

Patent ·
OSTI ID:872317
 [1];  [2]
  1. Miami, FL
  2. Los Alamos, NM
+ Show Author Affiliations

Time-resolved fluorescence decay measurements for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated cw laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes.

Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
DOE Contract Number:
W-7405-ENG-36
Assignee:
Regents of University of California (Los Alamos, NM)
Patent Number(s):
US 5909278
OSTI ID:
872317
Country of Publication:
United States
Language:
English

References (5)

Fluorescence detection and size measurement of single DNA molecules journal April 1993
Simultaneous dual-frequency phase-sensitive flow cytometric measurements for rapid identification of heterogeneous fluorescence decays in fluorochrome-labeled cells and particles journal December 1995
Time-resolved flow cytometry for the measurement of lanthanide chelate fluorescence: I. Concept and theoretical evaluation journal July 1994
Time-resolved fluorescence-decay measurement and analysis on single cells by flow cytometry journal January 1996
Time-resolved flow cytometry for the measurement of lanthanide chelate fluorescence: II. Instrument design and experimental results journal July 1994

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time-resolved
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decay
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flowing
particles
apparatus
method
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analysis
individual
cells
flow
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rapid
capabilities
cytometry
robust
procedures
time-domain
lifetime
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pulse-modulated
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excitation
characteristics
repetition
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individual cells
repetition rate
wide range
flow cytometry
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rapid measurement
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time-resolved fluorescence
fluorescence decay
flowing particles
decay measurements
lifetime spectroscopy
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