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Title: Monoterpene synthases from common sage (Salvia officinalis)

Patent ·
OSTI ID:872239
 [1];  [1];  [1];  [2]
  1. Pullman, WA
  2. Christchurch 5, NZ
+ Show Author Affiliations

cDNAs encoding (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase from common sage (Salvia officinalis) have been isolated and sequenced, and the corresponding amino acid sequences has been determined. Accordingly, isolated DNA sequences (SEQ ID No:1; SEQ ID No:3 and SEQ ID No:5) are provided which code for the expression of (+)-bornyl diphosphate synthase (SEQ ID No:2), 1,8-cineole synthase (SEQ ID No:4) and (+)-sabinene synthase SEQ ID No:6), respectively, from sage (Salvia officinalis). In other aspects, replicable recombinant cloning vehicles are provided which code for (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase, or for a base sequence sufficiently complementary to at least a portion of (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase may be used to obtain expression or enhanced expression of (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase in plants in order to enhance the production of monoterpenoids, or may be otherwise employed for the regulation or expression of (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase, or the production of their products.

Research Organization:
Washington State University Research Foundation (Pullman, WA)
DOE Contract Number:
FG03-96ER20212
Assignee:
Washington State University Research Foundation (Pullman, WA)
Patent Number(s):
US 5891697
OSTI ID:
872239
Country of Publication:
United States
Language:
English

References (15)

Isotopically sensitive branching in the formation of cyclic monoterpenes: proof that (-)-.alpha.-pinene and (-)-.beta.-pinene are synthesized by the same monoterpene cyclase via deprotonation of a common intermediate journal August 1987
Biosynthesis of monoterpenes: Preliminary characterization of bornyl pyrophosphate synthetase from sage (Salvia officinalis) and demonstration that geranyl pyrophosphate is the preferred substrate for cyclization journal December 1979
Isolation and Characterization of an Active-Site Peptide from a Monoterpene Cyclase Labeled with a Mechanism-Based Inhibitor journal February 1995
Biosynthesis of monoterpenes: Hydrolysis of bornyl pyrophosphate, an essential step in camphor biosynthesis, and hydrolysis of geranyl pyrophosphate, the acyclic precursor of camphor, by enzymes from sage (Salvia officinalis) journal December 1979
cDNA Cloning, Characterization, and Functional Expression of 4S-(−)-Limonene Synthase fromPerilla frutescens journal August 1996
Isotopically sensitive branching as a tool for evaluating multiple product formation by monoterpene cyclases journal July 1991
Monoterpene Biosynthesis book May 1992
4S-limonene synthase from the oil glands of spearmint (Mentha spicata). cDNA isolation, characterization, and bacterial expression of the catalytically active monoterpene cyclase. journal November 1993
Monoterpene Biosynthesis: Isotope Effects Associated with Bicyclic Olefin Formation Catalyzed by Pinene Synthases from Sage (Salvia officinalis) journal February 1994
Biosynthesis of Monoterpenes: Partial Purification, Characterization, and Mechanism of Action of 1,8-Cineole Synthase journal February 1994
Stereochemistry of the Proton Elimination in the Formation of (+)- and (−)-α-Pinene by Monoterpene Cyclases from Sage (Salvia officinalis) journal February 1994
Induced oleoresin biosynthesis in grand fir as a defense against bark beetles. journal May 1995
Pinene cyclases I and II. Two enzymes from sage (Salvia officinalis) which catalyze stereospecific cyclizations of geranyl pyrophosphate to monoterpene olefins of opposite configuration. journal January 1984
Biosynthesis of Monoterpenes journal February 1989
Monoterpene Synthases from Grand Fir ( Abies grandis ) : cDNA ISOLATION, CHARACTERIZATION, AND FUNCTIONAL EXPRESSION OF MYRCENE SYNTHASE, (−)-(4 journal August 1997

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isolated dna
significant amounts
acid sequence
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significant amount
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common sage
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