Method for the production of dicarboxylic acids
- Knoxville, TN
- Warrenville, IL
- Plainfield, IL
- Woodridge, IL
The present invention is an economical fermentation method for the production of carboxylic acids comprising the steps of a) inoculating a medium having a carbon source with a carboxylic acid-producing organism; b) incubating the carboxylic acid-producing organism in an aerobic atmosphere to promote rapid growth of the organism thereby increasing the biomass of the organism; c) controllably releasing oxygen to maintain the aerobic atmosphere; d) controllably feeding the organism having increased biomass with a solution containing the carbon source to maintain the concentration of the carbon source within the medium of about 0.5 g/L up to about 1 g/L; e) depriving the aerobic atmosphere of oxygen to produce an anaerobic atmosphere to cause the organism to undergo anaerobic metabolism; f) controllably feeding the organism having increased biomass a solution containing the carbon source to maintain the concentration of the carbon source within the medium of .gtoreq.1 g/L; and g) converting the carbon source to carboxylic acids using the anaerobic metabolism of the organism.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-31109-ENG-38; AC05-96OR22464
- Assignee:
- Lockhead Martin Energy Research Corporation (Oak Ridge, TN)
- Patent Number(s):
- US 5869301
- OSTI ID:
- 872139
- Country of Publication:
- United States
- Language:
- English
Carbon metabolism regulates expression of the pfl (pyruvate formate-lyase) gene in Escherichia coli.
|
journal | January 1991 |
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Related Subjects
production
dicarboxylic
acids
economical
fermentation
carboxylic
comprising
steps
inoculating
medium
carbon
source
acid-producing
organism
incubating
aerobic
atmosphere
promote
rapid
growth
increasing
biomass
controllably
releasing
oxygen
maintain
feeding
increased
solution
containing
concentration
depriving
produce
anaerobic
undergo
metabolism
gtoreq
converting
solution containing
carbon source
carboxylic acids
carboxylic acid
releasing oxygen
dicarboxylic acid
fermentation method
dicarboxylic acids
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