Flow cytometer jet monitor system
- Seattle, WA
A direct jet monitor illuminates the jet of a flow cytometer in a monitor wavelength band which is substantially separate from the substance wavelength band. When a laser is used to cause fluorescence of the substance, it may be appropriate to use an infrared source to illuminate the jet and thus optically monitor the conditions within the jet through a CCD camera or the like. This optical monitoring may be provided to some type of controller or feedback system which automatically changes either the horizontal location of the jet, the point at which droplet separation occurs, or some other condition within the jet in order to maintain optimum conditions. The direct jet monitor may be operated simultaneously with the substance property sensing and analysis system so that continuous monitoring may be achieved without interfering with the substance data gathering and may be configured so as to allow the front of the analysis or free fall area to be unobstructed during processing.
- Research Organization:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- DOE Contract Number:
- FG06-93ER61662
- Assignee:
- University of Washington ()
- Patent Number(s):
- US 5602039
- OSTI ID:
- 870824
- Country of Publication:
- United States
- Language:
- English
Operation of a Flow Cytometer
|
book | January 1992 |
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Related Subjects
cytometer
jet
monitor
direct
illuminates
wavelength
band
substantially
separate
substance
laser
fluorescence
appropriate
infrared
source
illuminate
optically
conditions
ccd
camera
optical
monitoring
provided
type
controller
feedback
automatically
changes
horizontal
location
droplet
separation
occurs
condition
maintain
optimum
operated
simultaneously
property
sensing
analysis
continuous
achieved
interfering
data
gathering
configured
allow
front
free
fall
unobstructed
processing
continuous monitoring
ccd camera
data gathering
flow cytometer
wavelength band
separation occurs
substantially separate
jet monitor
optical monitor
infrared source
optically monitor
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