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Title: Recombinant glucose uptake system

Patent ·
OSTI ID:870823
 [1];  [2];  [3]
  1. Gainesville, FL
  2. Groede, NL
  3. Delft, NL
+ Show Author Affiliations

Recombinant organisms are disclosed that contain a pathway for glucose uptake other than the pathway normally utilized by the host cell. In particular, the host cell is one in which glucose transport into the cell normally is coupled to PEP production. This host cell is transformed so that it uses an alternative pathway for glucose transport that is not coupled to PEP production. In a preferred embodiment, the host cell is a bacterium other than Z. mobilis that has been transformed to contain the glf and glk genes of Z. mobilis. By uncoupling glucose transport into the cell from PEP utilization, more PEP is produced for synthesis of products of commercial importance from a given quantity of biomass supplied to the host cells.

Research Organization:
Univ. of Florida, Gainesville, FL (United States)
DOE Contract Number:
FG05-86ER13574
Assignee:
University of Florida (Gainesville, FL)
Patent Number(s):
US 5602030
OSTI ID:
870823
Country of Publication:
United States
Language:
English

References (7)

Glucose transport in Escherichia coli journal June 1989
Cloning of glucose phosphorylating genes in S. cerevisiae by the KU-method and application to ATP production. journal January 1984
The Bacterial Phosphoenol-Pyruvate: Glycose Phosphotransferase System journal June 1990
Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase. journal January 1975
Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae. journal March 1983
Roles of multiple glucose transporters in Saccharomyces cerevisiae journal January 1993
Cloning of the glucokinase gene in Escherichia coli B journal November 1983

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+3 more
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Related Subjects

recombinant
glucose
uptake
organisms
disclosed
contain
pathway
normally
utilized
host
cell
particular
transport
coupled
pep
production
transformed
alternative
preferred
embodiment
bacterium
z
mobilis
glf
glk
genes
uncoupling
utilization
produced
synthesis
products
commercial
importance
quantity
biomass
supplied
cells
preferred embodiment
host cells
host cell
recombinant organisms
glucose uptake
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