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Title: Recombinant zymomonas for pentose fermentation

Patent ·
OSTI ID:870406
 [1];  [2];  [3];  [2];  [4]
  1. Golden, CO
  2. Lakewood, CO
  3. Littleton, CO
  4. Fort Collins, CO
+ Show Author Affiliations

The invention relates to microorganisms which normally do not ferment a pentose sugar and which are genetically altered to ferment this pentose to produce ethanol. A representative example is Zymomonas mobilis which has been transformed with E. coli xylose isomerase, xylulokinase, transaldolase and transketolase genes. Expression of the added genes are under the control of Zymomonas mobilis promoters. This newly created microorganism is useful for fermenting pentoses and glucose, produced by hydrolysis of hemicellulose and cellulose, to produce ethanol.

Research Organization:
Midwest Research Institute, Kansas City, MO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-83CH10093
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Number(s):
US 5514583
OSTI ID:
870406
Country of Publication:
United States
Language:
English

References (5)

Xylose fermentation by Saccharomyces cerevisiae journal March 1993
Isolation and characterization of the Pichia stipitis xylitol dehydrogenase gene, XYL2, and construction of a xylose-utilizing Saccharomyces cerevisiae transformant journal January 1990
Construction of xylose-assimilating Saccharomyces cerevisiae journal January 1993
Approaches to broaden the substrate and product range of the ethanologenic bacterium Zymomonas mobilis by genetic engineering journal February 1993
Systematic sequencing of the Escherichia coli genome: analysis of the 0 – 2.4 min region journal January 1992

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Related Subjects

recombinant
zymomonas
pentose
fermentation
relates
microorganisms
normally
ferment
sugar
genetically
altered
produce
ethanol
representative
example
mobilis
transformed
coli
xylose
isomerase
xylulokinase
transaldolase
transketolase
genes
expression
added
control
promoters
newly
created
microorganism
useful
fermenting
pentoses
glucose
produced
hydrolysis
hemicellulose
cellulose
xylose isomerase
pentose sugar
zymomonas mobilis
genetically altered
produce ethanol
recombinant zymomonas
pentose fermentation
newly created
mobilis promoters
added genes
fermenting pentoses
/435/999/