High speed flow cytometric separation of viable cells

Sasaki, Dennis T; Van den Engh, Gerrit J; Buckie, Anne-Marie

Title: High speed flow cytometric separation of viable cells

Abstract

Hematopoietic cell populations are separated to provide cell sets and subsets as viable cells with high purity and high yields, based on the number of original cells present in the mixture. High-speed flow cytometry is employed using light characteristics of the cells to separate the cells, where high flow speeds are used to reduce the sorting time.

Inventors:

Sasaki, Dennis T ^[1]; Van den Engh, Gerrit J ^[2]; Buckie, Anne-Marie ^[3]

Mountain View, CA
Seattle, WA
Margate, GB

Publication Date:: Sun Jan 01 00:00:00 EST 1995

Research Org.:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

OSTI Identifier:: 870155

Patent Number(s):: US 5466572

Assignee:: Systemix, Inc. (Palo Alto, CA)

DOE Contract Number:: W-7405-ENG-48

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: speed; flow; cytometric; separation; viable; cells; hematopoietic; cell; populations; separated; provide; sets; subsets; purity; yields; based; original; mixture; high-speed; cytometry; employed; light; characteristics; separate; speeds; reduce; sorting; time; flow cytometric; flow cytometry; viable cells; cell populations; speed flow; /435/436/

Citation Formats


                    Sasaki, Dennis T, Van den Engh, Gerrit J, and Buckie, Anne-Marie. High speed flow cytometric separation of viable cells.  United States: N. p., 1995. 
        Web.

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                    Sasaki, Dennis T, Van den Engh, Gerrit J, & Buckie, Anne-Marie. High speed flow cytometric separation of viable cells.  United States.

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                    Sasaki, Dennis T, Van den Engh, Gerrit J, and Buckie, Anne-Marie. 1995.  
        "High speed flow cytometric separation of viable cells".  United States.  https://www.osti.gov/servlets/purl/870155.

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@article{osti_870155,

  title        = {High speed flow cytometric separation of viable cells},

  author       = {Sasaki, Dennis T and Van den Engh, Gerrit J and Buckie, Anne-Marie},

  abstractNote = {Hematopoietic cell populations are separated to provide cell sets and subsets as viable cells with high purity and high yields, based on the number of original cells present in the mixture. High-speed flow cytometry is employed using light characteristics of the cells to separate the cells, where high flow speeds are used to reduce the sorting time.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/870155},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Sun Jan 01 00:00:00 EST 1995},

  month        = {Sun Jan 01 00:00:00 EST 1995}

}

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Works referenced in this record:

High-speed chromosome sorting
journal, October 1987

Gray, J.; Dean, P.; Fuscoe, J.
Science, Vol. 238, Issue 4825
https://doi.org/10.1126/science.2443974

The LLNL high-speed sorter: Design features, operational characteristics, and biological utility
journal, July 1985

Peters, D.; Branscomb, E.; Dean, P.
Cytometry, Vol. 6, Issue 4
https://doi.org/10.1002/cyto.990060404

Parallel processing data acquisition system for multilaser flow cytometry and cell sorting
journal, May 1989

van den Engh, Ger; Stokdijk, Willem
Cytometry, Vol. 10, Issue 3
https://doi.org/10.1002/cyto.990100307

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High speed flow cytometric separation of viable cells

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Hematopoietic cell populations are separated to provide cell sets and subsets as viable cells with high purity and high yields, based on the number of original cells present in the mixture. High-speed flow cytometry is employed using light characteristics of the cells to separate the cells, where high flow speeds are used to reduce the sorting time.
High speed flow cytometric detection of rare glycophorin A mutations in human blood cells

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Flow cytometric analysis and sorting of chromosomes

Conference Van Dilla, M; Dean, P; Fuscoe, J; ...

For analysis by flow cytometry, metaphase chromosomes are isolated from mitotic cells, and then stained while in suspension with one or more usually two, DNA-specific fluorescent dyes. The chromosomes are then processed one at a time through a flow cytometer or sorter where their dye content is measured at high speed and with high precision. In the work described here, dual staining is used since bivariate analysis affords better discrimination among chromosomal types: two dyes commonly used are Hoechst 33258, which binds preferentially to AT-rich DNA, and chromomycin A3, which bind preferentially to GC-rich DNA. For bivariate flow studies themore »« less
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A bacterial neutral protease was used to disperse KHT solid tumors into single cell suspensions suitable for routine cell kinetic analysis by flow cytometry and for clonogenic cell survival. Neutral protease disaggregation under conditions which would be suitable for routine tumor dispersal was compared with a trypsin/DNase procedure. Cell yield, clonogenic cell survival, DNA distributions of untreated and drug-perturbed tumors, rates of radioactive precursor incorporation during the cell cycle, and preferential cell cycle phase-specific cell loss were investigated. Tumors dispersed with neutral protease yielded approximately four times more cells than those dispersed with trypsin/DNase and approximately a 1.5-fold higher platingmore »« less
Flow cytometry

Journal Article Steinkamp, J - Rev. Sci. Instrum.; (United States)

Flow cytometry instrumentation developed from early efforts to count cells and particles in liquid suspension as they passed through a sensing device. Since the mid-1960's sophisticated instruments have been designed for analyzing cells based on various cytological, biochemical, and functional properties. These instruments have revolutionized automated cell analysis methods in that measurements are made at high speed, multiparameter data is correlated on each cell, statistical precision is high, and cells are separated in high purity from heterogeneous mixtures for identification and functional analysis. Advanced instruments capable of measuring cell volume, surface area, multicolor fluorescence, fluorescence polarization, light scatter within variousmore »« less
https://doi.org/10.1063/1.1137948

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Title: High speed flow cytometric separation of viable cells

Abstract

Citation Formats

High-speed chromosome sorting journal, October 1987

The LLNL high-speed sorter: Design features, operational characteristics, and biological utility journal, July 1985

Parallel processing data acquisition system for multilaser flow cytometry and cell sorting journal, May 1989

High-speed chromosome sorting
journal, October 1987

The LLNL high-speed sorter: Design features, operational characteristics, and biological utility
journal, July 1985

Parallel processing data acquisition system for multilaser flow cytometry and cell sorting
journal, May 1989