Catalytic and reactive polypeptides and methods for their preparation and use
Abstract
Catalytic and reactive polypeptides include a binding site specific for a reactant or reactive intermediate involved in a chemical reaction of interest. The polypeptides further include at least one active functionality proximate the binding site, where the active functionality is capable of catalyzing or chemically participating in the chemical reaction in such a way that the reaction rate is enhanced. Methods for preparing the catalytic peptides include chemical synthesis, site-directed mutagenesis of antibody and enzyme genes, covalent attachment of the functionalities through particular amino acid side chains, and the like.
- Inventors:
-
- Oakland, CA
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- OSTI Identifier:
- 869234
- Patent Number(s):
- US 5302516
- Assignee:
- Regents of University of California (Berkeley, CA)
- DOE Contract Number:
- C87-101226
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- catalytic; reactive; polypeptides; methods; preparation; binding; site; specific; reactant; intermediate; involved; chemical; reaction; active; functionality; proximate; capable; catalyzing; chemically; participating; rate; enhanced; preparing; peptides; synthesis; site-directed; mutagenesis; antibody; enzyme; genes; covalent; attachment; functionalities; particular; amino; acid; chains; reactive polypeptides; chemical synthesis; active functionality; reaction rate; binding site; amino acid; chemical reaction; reactive intermediate; site specific; functionality proximate; intermediate involved; active poly; active polypeptide; /435/
Citation Formats
Schultz, Peter. Catalytic and reactive polypeptides and methods for their preparation and use. United States: N. p., 1994.
Web.
Schultz, Peter. Catalytic and reactive polypeptides and methods for their preparation and use. United States.
Schultz, Peter. 1994.
"Catalytic and reactive polypeptides and methods for their preparation and use". United States. https://www.osti.gov/servlets/purl/869234.
@article{osti_869234,
title = {Catalytic and reactive polypeptides and methods for their preparation and use},
author = {Schultz, Peter},
abstractNote = {Catalytic and reactive polypeptides include a binding site specific for a reactant or reactive intermediate involved in a chemical reaction of interest. The polypeptides further include at least one active functionality proximate the binding site, where the active functionality is capable of catalyzing or chemically participating in the chemical reaction in such a way that the reaction rate is enhanced. Methods for preparing the catalytic peptides include chemical synthesis, site-directed mutagenesis of antibody and enzyme genes, covalent attachment of the functionalities through particular amino acid side chains, and the like.},
doi = {},
url = {https://www.osti.gov/biblio/869234},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 1994},
month = {Sat Jan 01 00:00:00 EST 1994}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Antibody Catalysis by Transition State Stabilization
journal, January 1987
- Pollack, S. J.; Schultz, P. G.
- Cold Spring Harbor Symposia on Quantitative Biology, Vol. 52, Issue 0
Designer catalytic antibodies
journal, March 1989
- Leatherbarrow, Robin J.
- Nature, Vol. 338, Issue 6212
A semisynthetic catalytic antibody
journal, March 1989
- Pollack, Scott J.; Schultz, Peter G.
- Journal of the American Chemical Society, Vol. 111, Issue 5
Catalysis of concerted reactions by antibodies: the Claisen rearrangement.
journal, July 1988
- Hilvert, D.; Carpenter, S. H.; Nared, K. D.
- Proceedings of the National Academy of Sciences, Vol. 85, Issue 14
Transaminations by pyridoxamine selectively attached at C-3 in .beta.-cyclodextrin
journal, March 1983
- Breslow, Ronald; Czarnik, Anthony W.
- Journal of the American Chemical Society, Vol. 105, Issue 5
Principles of antibody catalysis
journal, October 1988
- Lerner, Richard A.; Benkovic, Stephen J.
- BioEssays, Vol. 9, Issue 4
Antibody-enzyme analogy. Characterization of antibodies to phosphopyridoxyltyrosine derivatives
journal, February 1975
- Raso, Vic; Stollar, B. D.
- Biochemistry, Vol. 14, Issue 3
Engineering antibody affinity and specificity
journal, January 1988
- Webster, D. M.; Roberts, S.; Cheetham, J. C.
- International Journal of Cancer, Vol. 41, Issue S3
A-(modified B6)-B-[.omega.-amino(ethylamino)]-.beta.-cyclodextrin as an artificial B6 enzyme for chiral aminotransfer reaction
journal, September 1985
- Tabushi, Iwao; Kuroda, Yasuhisa; Yamada, Masahiko
- Journal of the American Chemical Society, Vol. 107, Issue 19
Intramolecular general base-acid catalysis in transaminations catalyzed by pyridoxamine enzyme analogs
journal, March 1983
- Zimmerman, Steven C.; Czarnik, Anthony W.; Breslow, Ronald
- Journal of the American Chemical Society, Vol. 105, Issue 6
Induction of an antibody that catalyzes the hydrolysis of an amide bond
journal, September 1988
- Janda, K.; Schloeder, D.; Benkovic, S.
- Science, Vol. 241, Issue 4870
Catalytic antibodies
journal, April 1987
- Jacobs, Jeffrey; Schultz, Peter G.; Sugasawara, Renee
- Journal of the American Chemical Society, Vol. 109, Issue 7
Host-guest complexation. 31. A transacylase partial mimic
journal, August 1984
- Cram, Donald J.; Katz, Howard Edan; Dicker, Ira B.
- Journal of the American Chemical Society, Vol. 106, Issue 17
Catalysis and chiral recognition through designed complexation of transition states in transacylations of amino ester salts
journal, February 1976
- Chao, Y.; Cram, Donald J.
- Journal of the American Chemical Society, Vol. 98, Issue 4
Molecular catalysis: enhanced rates of thiolysis with high structural and chiral recognition in complexes of a reactive macrocyclic receptor molecule
journal, January 1978
- Lehn, Jean-Marie; Sirlin, Claude
- Journal of the Chemical Society, Chemical Communications, Issue 21
Reversible oxygenation and autoxidation of a capped porphyrin iron(II) complex
journal, January 1975
- Almog, Joseph; Baldwin, Jack E.; Huff, Joel
- Journal of the American Chemical Society, Vol. 97, Issue 1
Antibody catalysis approaching the activity of enzymes
journal, March 1988
- Tramontano, Alfonso.; Ammann, Adrian A.; Lerner, Richard A.
- Journal of the American Chemical Society, Vol. 110, Issue 7
Generation of a hybrid sequence-specific single-stranded deoxyribonuclease
journal, December 1987
- Corey, D.; Schultz, P.
- Science, Vol. 238, Issue 4832
A stereospecific cyclization catalyzed by an antibody
journal, August 1987
- Napper, A.; Benkovic, S.; Tramontano, A.
- Science, Vol. 237, Issue 4818
The conversion of serine at the active site of subtilisin to cysteine: a "chemical mutation".
journal, November 1966
- Neet, K. E.; Koshland, D. E.
- Proceedings of the National Academy of Sciences, Vol. 56, Issue 5
Catalysis of a stereospecific bimolecular amide synthesis by an antibody.
journal, August 1988
- Benkovic, S. J.; Napper, A. D.; Lerner, R. A.
- Proceedings of the National Academy of Sciences, Vol. 85, Issue 15
Cyclodextrin catalysis as a model for enzyme action
journal, March 1982
- Tabushi, Iwao
- Accounts of Chemical Research, Vol. 15, Issue 3
Antibody catalysis of bimolecular amide formation
journal, July 1988
- Janda, K. D.; Lerner, R. A.; Tramontano, Alfonso.
- Journal of the American Chemical Society, Vol. 110, Issue 14
Chemical mutation of enzyme active sites
journal, November 1984
- Kaiser, E.; Lawrence, D.
- Science, Vol. 226, Issue 4674
Photosensitized cleavage of a thymine dimer by an antibody
journal, November 1988
- Cochran, Andrea G.; Sugasawara, Renee.; Schultz, Peter G.
- Journal of the American Chemical Society, Vol. 110, Issue 23
Antibodies as enzymes
journal, January 1987
- Lerner, Richard A.; Tramontano, Alfonso
- Trends in Biochemical Sciences, Vol. 12
Purification and properties of thiol beta-lactamase. A mutant of pBR322 beta-lactamase in which the active site serine has been replaced with cysteine.
journal, April 1984
- Sigal, I. S.; DeGrado, W. F.; Thomas, B. J.
- Journal of Biological Chemistry, Vol. 259, Issue 8
A new strategy for the generation of catalytic antibodies
journal, March 1989
- Shokat, K. M.; Leumann, C. J.; Sugasawara, R.
- Nature, Vol. 338, Issue 6212
An antibody-catalyzed Claisen rearrangement
journal, July 1988
- Jackson, D. Y.; Jacobs, J. W.; Sugasawara, R.
- Journal of the American Chemical Society, Vol. 110, Issue 14
Synthesis of the Myoglobin Active Site
journal, September 1973
- Chang, C. K.; Traylor, T. G.
- Proceedings of the National Academy of Sciences, Vol. 70, Issue 9
Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.
journal, November 1982
- Dalbadie-McFarland, G.; Cohen, L. W.; Riggs, A. D.
- Proceedings of the National Academy of Sciences, Vol. 79, Issue 21
Synthetic models for the oxygen-binding hemoproteins
journal, July 1977
- Collman, James P.
- Accounts of Chemical Research, Vol. 10, Issue 7
Catalytic antibodies
journal, December 1986
- Tramontano, A.; Janda, K.; Lerner, R.
- Science, Vol. 234, Issue 4783
Directed mutagenesis of the redox-active disulfide in the flavoenzyme mercuric ion reductase
journal, November 1985
- Schultz, Peter G.; Au, Karin G.; Walsh, Christopher T.
- Biochemistry, Vol. 24, Issue 24
Selective chemical catalysis by an antibody
journal, December 1986
- Pollack, S.; Jacobs, J.; Schultz, P.
- Science, Vol. 234, Issue 4783
Introduction of nucleophiles and spectroscopic probes into antibody combining sites
journal, November 1988
- Pollack, S.; Nakayama, G.; Schultz, P.
- Science, Vol. 242, Issue 4881
Localization of affinity-labeled residues on the heavy and light chain of two myeloma proteins with anti-hapten activity
journal, June 1972
- Haimovich, J.; Eisen, H. N.; Hurwitz, E.
- Biochemistry, Vol. 11, Issue 13
Redesigning trypsin: alteration of substrate specificity
journal, April 1985
- Craik, C.; Largman, C.; Fletcher, T.
- Science, Vol. 228, Issue 4697
Monoclonal immunoglobulin G augments hydrolysis of an ester of the homologous hapten: An esterase-like activity of the antibody-containing site?
journal, March 1980
- Kohen, F.; Kim, J. B.; Lindner, H. R.
- FEBS Letters, Vol. 111, Issue 2
A large increase in enzyme–substrate affinity by protein engineering
journal, January 1984
- Wilkinson, Anthony J.; Fersht, Alan R.; Blow, D. M.
- Nature, Vol. 307, Issue 5947
Linkage of cytotoxic agents to immunoglobulins
journal, January 1983
- Huntley Blair, A.; Ghose, Tarun I.
- Journal of Immunological Methods, Vol. 59, Issue 2
A New Enzyme Containing a Synthetically Formed Active Site. Thiol-Subtilisin 1
journal, July 1966
- Polgar, Laszlo; Bender, Myron L.
- Journal of the American Chemical Society, Vol. 88, Issue 13
Chemical reactivity at an antibody binding site elicited by mechanistic design of a synthetic antigen.
journal, September 1986
- Tramontano, A.; Janda, K. D.; Lerner, R. A.
- Proceedings of the National Academy of Sciences, Vol. 83, Issue 18
Affinity labeling of a mouse myeloma protein which binds nitrophenyl ligands. Kinetics of labeling and isolation of a labeled peptide
journal, March 1970
- Goetzl, Edward J.; Metzger, Henry
- Biochemistry, Vol. 9, Issue 5
The interplay between chemistry and biology in the design of enzymatic catalysts
journal, April 1988
- Schultz, P.
- Science, Vol. 240, Issue 4851
Eine über Antikörper gesteuerte Redoxreaktion
journal, September 1988
- Shokat, Kevan M.; Leumann, Christian H.; Sugasawara, Renee
- Angewandte Chemie, Vol. 100, Issue 9
Affinity labeling and cross-linking of the heavy and light chains of a myeloma protein with anti-2,4-dinitrophenol activity
journal, August 1971
- Givol, D.; Strausbauch, P. H.; Hurwitz, E.
- Biochemistry, Vol. 10, Issue 18