skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Lectin cDNA and transgenic plants derived therefrom

Patent ·
OSTI ID:869114

Transgenic plants containing cDNA encoding Gramineae lectin are described. The plants preferably contain cDNA coding for barley lectin and store the lectin in the leaves. The transgenic plants, particularly the leaves exhibit insecticidal and fungicidal properties. GOVERNMENT RIGHTS This application was funded under Department of Energy Contract DE-AC02-76ER01338. The U.S. Government has certain rights under this application and any patent issuing thereon.

Research Organization:
Michigan State Univ., East Lansing, MI (United States)
DOE Contract Number:
AC02-76ER01338
Assignee:
Board of Trustees operating Michigan State University (East Lansing, MI)
Patent Number(s):
US 5276269
Application Number:
07/917,665
OSTI ID:
869114
Country of Publication:
United States
Language:
English

References (35)

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity journal July 1983
A genetic basis for the origin of six different isolectins in hexaploid wheat journal June 1982
Insecticidal Activity and Lectin Homology of Arcelin Seed Protein journal April 1988
Direct Evidence for a Sugar Transport Mechanism in Isolated Vacuoles journal July 1979
Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP journal January 1986
Optimizing hydrolysis of N-linked high-mannose oligosaccharides by endo-β-N-acetylglucosaminidase H journal September 1984
Isolation and characterization of a cDNA clone encoding wheat germ agglutinin journal October 1987
Expression of rice lectin is governed by two temporally and spatially regulated mRNAs in developing embryos. journal May 1989
Different properties of two types of auxin-binding sites in membranes from maize coleoptiles journal October 1988
Agrobacterium molecular genetics book January 1994
Biosynthesis of lectin in roots of germinating and adult cereal plants journal January 1985
DNA sequencing with chain-terminating inhibitors journal December 1977
Plant Lectins: Molecular and Biological Aspects journal June 1985
A simple and very efficient method for generating cDNA libraries journal November 1983
[15] A rapid single-stranded cloning, sequencing, insertion, and deletion strategy book January 1987
A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots journal January 1984
Immunocytochemical localization of wheat germ agglutinin in wheat. journal March 1982
Timing, localization, and control of wheat germ agglutinin synthesis in developing wheat embryos journal June 1982
Sequence complexity of polyadenylated ribonucleic acid from soybean suspension culture cells journal June 1979
Binary vectors book January 1989
Interference by Phosphatases in the Spectrophotometric Assay for Phospho enol pyruvate Carboxylase journal March 1989
A plant DNA minipreparation: Version II journal September 1983
Hybrid Methods in Pattern Recognition book January 1987
Cell-type-specific expression of a wheat-germ agglutinin gene in embryos and young seedlings of Triticum aestivum journal December 1988
A new method for predicting signal sequence cleavage sites journal January 1986
Bacillus thuringiensis §-Endotoxin Expressed in Transgenic Nicotiana tabacum Provides Resistance to Lepidopteran Insects journal December 1987
Localization of Wheat Germ Agglutinin--Like Lectins in Various Species of the Gramineae journal June 1983
Characterization of a wheat germ agglutinin-like lectin from adult wheat plants journal September 1984
Light-stimulated accumulation of the peroxisomal enzymes hydroxypyruvate reductase and serine:glyoxylate aminotransferase and their translatable mRNAs in cotyledons of cucumber seedlings journal January 1987
Occurrence and immunological relationships of lectins in gramineous species journal October 1983
Wheat-germ agglutinin is synthesized as a glycosylated precursor journal January 1988
Rapeseed Embryo Development in Culture on High Osmoticum Is Similar to That in Seeds journal July 1986
Hydrolytic Enzymes in the Central Vacuole of Plant Cells journal June 1979
Biological effects of plant lectins on the cowpea weevil journal January 1990
Evidence for N- and C-terminal processing of a plant defense-related enzyme: Primary structure of tobacco prepro- -1,3-glucanase journal August 1988