Catalyzed enzyme electrodes

Patent · Fri Jan 01 00:00:00 EST 1993

OSTI ID:868851

Zawodzinski, Thomas A ^[1]; Wilson, Mahlon S ^[1]; Rishpon, Judith ^[2]; Gottesfeld, Shimshon ^[1]

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1. Los Alamos, NM
2. Ramat-Aviv, IL

An enzyme electrode is prepared with a composite coating on an electrical conductor. The composite coating is formed from a casting solution of a perfluorosulfonic acid polymer, an enzyme, and a carbon supported catalyst. The solution may be cast directly on the conductor surface or may be formed as a membrane and applied to the surface. The perfluorosulfonic acid ionomer formed from the casting solution provides an insoluble biocompatible protective matrix for the enzyme and acts to retain the enzyme for long term availability in the electrode structure. The carbon supported catalyst provides catalytic sites throughout the layer for the oxidation of hydrogen peroxide from the enzyme reactions. The carbon support then provides a conductive path for establishing an electrical signal to the electrical conductor. In one embodiment, the electrical conductor is a carbon cloth that permits oxygen or other gas to be introduced to the perfluorosulfonic polymer to promote the enzyme reaction independent of oxygen in the solution being tested.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 5227042
• OSTI ID: 868851
• Country of Publication: United States
• Language: English

Related Subjects

catalyzed
enzyme
electrodes
electrode
prepared
composite
coating
electrical
conductor
formed
casting
solution
perfluorosulfonic
acid
polymer
carbon
supported
catalyst
cast
directly
surface
membrane
applied
ionomer
provides
insoluble
biocompatible
protective
matrix
retain
term
availability
structure
catalytic
sites
throughout
layer
oxidation
hydrogen
peroxide
reactions
support
conductive
path
establishing
signal
embodiment
cloth
permits
oxygen
gas
introduced
promote
reaction
independent
tested
acid polymer
composite coating
perfluorosulfonic acid
hydrogen peroxide
sulfonic acid
carbon cloth
electrode structure
electrical signal
electrical conductor
supported catalyst
conductive path
casting solution
solution provides
acid ionomer
carbon support
carbon supported
cast directly
provides catalytic
enzyme electrode
catalyst provide
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