Regioselective chemical modification of monoclonal antibodies

Ranadive, Girish; Rosenzweig, Howard S; Epperly, Michael; Bloomer, William

Title: Regioselective chemical modification of monoclonal antibodies

Abstract

A method of selectively modifying an immunoglobulin having at least one Fab region and at least one Fc region, each region having an isoelectric point wherein said isoelectric point of the Fab fragment of said immunoglobulin is different than the isoelectric point of the Fc fragment of the immunoglobulin, said method comprising modification of the immunoglobulin at a pH between the respective isoelectric points of the Fab and Fc fragments of the immunoglobulin.

Inventors:

Ranadive, Girish ^[1]; Rosenzweig, Howard S ^[1]; Epperly, Michael ^[1]; Bloomer, William ^[1]

Pittsburgh, PA

Publication Date:: Fri Jan 01 00:00:00 EST 1993

Research Org.:: Univ. of Pittsburgh, PA (United States)

OSTI Identifier:: 868770

Patent Number(s):: US 5208008

Assignee:: University of Pittsburgh (Pittsburgh, PA)

DOE Contract Number:: FG02-89ER60869

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: regioselective; chemical; modification; monoclonal; antibodies; method; selectively; modifying; immunoglobulin; fab; region; fc; isoelectric; fragment; comprising; ph; respective; fragments; method comprising; monoclonal antibodies; selective chemical; selectively modifying; chemical modification; /424/435/436/530/

Citation Formats


                    Ranadive, Girish, Rosenzweig, Howard S, Epperly, Michael, and Bloomer, William. Regioselective chemical modification of monoclonal antibodies.  United States: N. p., 1993. 
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                    Ranadive, Girish, Rosenzweig, Howard S, Epperly, Michael, & Bloomer, William. Regioselective chemical modification of monoclonal antibodies.  United States.

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                    Ranadive, Girish, Rosenzweig, Howard S, Epperly, Michael, and Bloomer, William. 1993.  
        "Regioselective chemical modification of monoclonal antibodies".  United States.  https://www.osti.gov/servlets/purl/868770.

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@article{osti_868770,

  title        = {Regioselective chemical modification of monoclonal antibodies},

  author       = {Ranadive, Girish and Rosenzweig, Howard S and Epperly, Michael and Bloomer, William},

  abstractNote = {A method of selectively modifying an immunoglobulin having at least one Fab region and at least one Fc region, each region having an isoelectric point wherein said isoelectric point of the Fab fragment of said immunoglobulin is different than the isoelectric point of the Fc fragment of the immunoglobulin, said method comprising modification of the immunoglobulin at a pH between the respective isoelectric points of the Fab and Fc fragments of the immunoglobulin.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/868770},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Fri Jan 01 00:00:00 EST 1993},

  month        = {Fri Jan 01 00:00:00 EST 1993}

}

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Works referenced in this record:

Inhibition of mannosidase in hybridomas yields monoclonal antibodies with greater capacity for carbohydrate labeling.
journal, September 1988

Simonson, R. B.; Ultee, M. E.; Long, C. G.
Clinical Chemistry, Vol. 34, Issue 9
https://doi.org/10.1093/clinchem/34.9.1709

Linkage of cytotoxic agents to immunoglobulins
journal, January 1983

Huntley Blair, A.; Ghose, Tarun I.
Journal of Immunological Methods, Vol. 59, Issue 2
https://doi.org/10.1016/0022-1759(83)90024-8

Strategies for designing antibody-toxin conjugates
journal, October 1988

Ahmad, Ateeq; Law, Kevin
Trends in Biotechnology, Vol. 6, Issue 10
https://doi.org/10.1016/0167-7799(88)90056-X

Monoclonal antibodies with cytotoxic reactivities against human gliomas
journal, December 1989

Nanda, Anil; Liwnicz, Boleslaw; Atkinson, Barbara F.
Journal of Neurosurgery, Vol. 71, Issue 6
https://doi.org/10.3171/jns.1989.71.6.0892

[20] Preparation of antibody-linked cytotoxic agents
book, January 1983

Ghose, Tarun I.; Huntley Blair, A.; Kulkarni, Padmaja N.
Immunochemical Techniques Part F: Conventional Antibodies, Fc Receptors, and Cytotoxicity
https://doi.org/10.1016/S0076-6879(83)93050-1

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Similar Records

Title: Regioselective chemical modification of monoclonal antibodies

Abstract

Citation Formats

Inhibition of mannosidase in hybridomas yields monoclonal antibodies with greater capacity for carbohydrate labeling. journal, September 1988

Linkage of cytotoxic agents to immunoglobulins journal, January 1983

Strategies for designing antibody-toxin conjugates journal, October 1988

Monoclonal antibodies with cytotoxic reactivities against human gliomas journal, December 1989

[20] Preparation of antibody-linked cytotoxic agents book, January 1983

Inhibition of mannosidase in hybridomas yields monoclonal antibodies with greater capacity for carbohydrate labeling.
journal, September 1988

Linkage of cytotoxic agents to immunoglobulins
journal, January 1983

Strategies for designing antibody-toxin conjugates
journal, October 1988

Monoclonal antibodies with cytotoxic reactivities against human gliomas
journal, December 1989

[20] Preparation of antibody-linked cytotoxic agents
book, January 1983