Multiplex analysis of DNA

Patent · Wed Jan 01 00:00:00 EST 1992

OSTI ID:868474

Church, George M ^[1]; Kieffer-Higgins, Stephen ^[2]

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1. Boston, MA
2. Dorchester, MA

This invention features vectors and a method for sequencing DNA. The method includes the steps of: a) ligating the DNA into a vector comprising a tag sequence, the tag sequence includes at least 15 bases, wherein the tag sequence will not hybridize to the DNA under stringent hybridization conditions and is unique in the vector, to form a hybrid vector, b) treating the hybrid vector in a plurality of vessels to produce fragments comprising the tag sequence, wherein the fragments differ in length and terminate at a fixed known base or bases, wherein the fixed known base or bases differs in each vessel, c) separating the fragments from each vessel according to their size, d) hybridizing the fragments with an oligonucleotide able to hybridize specifically with the tag sequence, and e) detecting the pattern of hybridization of the tag sequence, wherein the pattern reflects the nucleotide sequence of the DNA.

• Research Organization: Harvard University
• DOE Contract Number: FG02-87ER60565
• Assignee: President and Fellows of Harvard College (Cambridge, MA)
• Patent Number(s): US 5149625
• OSTI ID: 868474
• Country of Publication: United States
• Language: English

Related Subjects

multiplex
analysis
dna
features
vectors
method
sequencing
steps
ligating
vector
comprising
tag
sequence
15
bases
hybridize
stringent
hybridization
conditions
unique
form
hybrid
treating
plurality
vessels
produce
fragments
length
terminate
fixed
base
differs
vessel
separating
according
size
hybridizing
oligonucleotide
specifically
detecting
pattern
reflects
nucleotide
tag sequence
sequencing dna
nucleotide sequence
vector comprising
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