Nucleic acid isolation process
- Los Alamos, NM
- La Jolla, CA
A method is provided for isolating DNA from eukaryotic cell and flow sorted chromosomes. When DNA is removed from chromosome and cell structure, detergent and proteolytic digestion products remain with the DNA. These products can be removed with organic extraction, but the process steps associated with organic extraction reduce the size of DNA fragments available for experimental use. The present process removes the waste products by dialyzing a solution containing the DNA against a solution containing polyethylene glycol (PEG). The waste products dialyze into the PEG leaving isolated DNA. The remaining DNA has been prepared with fragments containing more than 160 kb. The isolated DNA has been used in conventional protocols without affect on the protocol.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- DOE Contract Number:
- W-7405-ENG-36
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Number(s):
- US 4921952
- OSTI ID:
- 867364
- Country of Publication:
- United States
- Language:
- English
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Nucleic acid isolation process
New and rapid procedure for the isolation of ultra-high molecular weight eukaryotic DNA
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acid
isolation
process
method
provided
isolating
dna
eukaryotic
cell
flow
sorted
chromosomes
removed
chromosome
structure
detergent
proteolytic
digestion
products
remain
organic
extraction
steps
associated
reduce
size
fragments
available
experimental
removes
waste
dialyzing
solution
containing
polyethylene
glycol
peg
dialyze
leaving
isolated
remaining
prepared
160
kb
conventional
protocols
affect
protocol
waste product
cell structure
ethylene glycol
polyethylene glycol
isolated dna
solution containing
nucleic acid
dna fragments
waste products
dna fragment
organic extraction
containing poly
process removes
process step
flow sorted
process steps
acid isolation
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