Tracking chemical changes in a live cell: Biomedical applications of SR-FTIR spectromicroscopy
Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectromicroscopy is a newly emerging bioanalytical and imaging tool. This unique technique provides mid-infrared (IR) spectra, hence chemical information, with high signal-to-noise at spatial resolutions as fine as 3 to 10 microns. Thus it enables researchers to locate, identify, and track specific chemical events within an individual living mammalian cell. Mid-IR photons are too low in energy (0.05 - 0.5 eV) to either break bonds or to cause ionization. In this review, we show that the synchrotron IR beam has no detectable effects on the short- and long-term viability, reproductive integrity, cell-cycle progression, and mitochondrial metabolism in living human cells, and produces only minimal sample heating (< 0.5 degrees C). We will then present several examples demonstrating the application potentials of SR-FTIR spectromicroscopy in biomedical research. These will include monitoring living cells progressing through the cell cycle, including death, and cells reacting to dilute concentrations of toxins.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE Director. Office of Science. Office of Biological and Environmental Research. Medical Science Division, National Petroleum Technology Office. National Petroleum Technology Program, Office of Basic Energy Sciences, Materials Science and Engineering Division; U.S. Department of Defense. Army Corps of Engineers, U.S. National Aeronautics and Space Administration (US)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 834624
- Report Number(s):
- LBNL-51337; R&D Project: 731201; TRN: US0407287
- Resource Relation:
- Conference: First International Conference on Biomedical Spectroscopy. From Molecules to Men, Cardiff, Wales (GB), 07/07/2002--07/10/2002; Other Information: PBD: 25 Jul 2002
- Country of Publication:
- United States
- Language:
- English
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