Development And Evaluation Of Stable Isotope And Fluorescent Labeling And Detection Methodologies For Tracking Injected Bacteria During In Situ Bioremediation

Technical Report · Wed Dec 17 00:00:00 EST 2003

DOI:https://doi.org/10.2172/820180· OSTI ID:820180

Fuller, Mark E; Onstott, Tullis C

This report summarizes the results of a research project conducted to develop new methods to label bacterial cells so that they could be tracked and enumerated as they move in the subsurface after they are introduced into the groundwater (i.e., during bioaugmentation). Labeling methods based on stable isotopes of carbon (13C) and vital fluorescent stains were developed. Both approaches proved successful with regards to the ability to effectively label bacterial cells. Several methods for enumeration of fluorescently-labeled cells were developed and validated, including near-real time microplate spectrofluorometry that could be performed in the field. However, the development of a novel enumeration method for the 13C-enriched cells, chemical reaction interface/mass spectrometry (CRIMS), was not successful due to difficulties with the proposed instrumentation. Both labeling methodologies were successfully evaluated and validated during laboratory- and field-scale bacterial transport experiments. The methods developed during this research should be useful for future bacterial transport work as well as other microbial ecology research in a variety of environments. A full bibliography of research articles and meeting presentations related to this project is included (including web links to abstracts and full text reprints).

• Research Organization: Shaw Environmental, Inc. (US)
• Sponsoring Organization: USDOE Office of Science (SC); USDOE Office of Science (SC) (US)
• DOE Contract Number: FG02-98ER62712
• OSTI ID: 820180
• Report Number(s): DOE/ER62712-1; TRN: US200411%%753
• Resource Relation: Other Information: PBD: 17 Dec 2003
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
58 GEOSCIENCES
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
BACTERIA
BIOREMEDIATION
CARBON
CHEMICAL REACTIONS
DETECTION
ECOLOGY
EVALUATION
SPECTROSCOPY
STABLE ISOTOPES
STAINS
TRANSPORT
BACTERIAL TRANSPORT
SUBSURFACE
VITAL FLUORESCENT STAIN
BIOAUGMENTATION
GEOMICROBIOLOGY
MICROBIAL ECOLOGY
STABLE CARBON ISOTOPE
SPECTROFLUOROMETRY
COLLOID TRANSPORT