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Title: Progress Report: DE-FG03-97ER20274, ''Microbial Production of Isoprene''

Abstract

We have discovered that microorganisms produce and emit the hydrocarbon isoprene (2-methyl-1,3-butadiene), and have suggested that if isoprene-producing enzymes and their genes can be harnessed, useful hydrocarbon-producing systems might be constructed. The main goal of the proposed work is to establish the biochemical mechanism and regulation of isoprene formation in the bacterial system, Bacillus subtilis. Specific objectives of the proposed work are the following: (A) to characterize the physiological regulation of isoprene formation in B. subtilis; (B) to characterize mutations in B. subtilis 168 that suppress isoprene formation, clone these genes, and determine how isoprene and isoprenoid carbon flow are regulated; and (C) to test ''overflow'' and ''signaling'' models for Bacillus isoprene formation. We are also pursuing the isolation and cloning of B. subtilis isoprene synthase, which we believe may be a regulatory enzyme.

Authors:
Publication Date:
Research Org.:
University of Colorado (US)
Sponsoring Org.:
USDOE Office of Energy Research (ER) (US)
OSTI Identifier:
792551
Report Number(s):
DOEER20274
TRN: US200212%%188
DOE Contract Number:  
FG03-97ER20274
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 13 Mar 2002
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; BACILLUS SUBTILIS; CLONING; ENZYMES; GENES; ISOPRENE; PROGRESS REPORT; BIOSYNTHESIS; BIOCHEMICAL REACTION KINETICS; MICROBIAL

Citation Formats

Fall, Ray. Progress Report: DE-FG03-97ER20274, ''Microbial Production of Isoprene''. United States: N. p., 2002. Web. doi:10.2172/792551.
Fall, Ray. Progress Report: DE-FG03-97ER20274, ''Microbial Production of Isoprene''. United States. https://doi.org/10.2172/792551
Fall, Ray. 2002. "Progress Report: DE-FG03-97ER20274, ''Microbial Production of Isoprene''". United States. https://doi.org/10.2172/792551. https://www.osti.gov/servlets/purl/792551.
@article{osti_792551,
title = {Progress Report: DE-FG03-97ER20274, ''Microbial Production of Isoprene''},
author = {Fall, Ray},
abstractNote = {We have discovered that microorganisms produce and emit the hydrocarbon isoprene (2-methyl-1,3-butadiene), and have suggested that if isoprene-producing enzymes and their genes can be harnessed, useful hydrocarbon-producing systems might be constructed. The main goal of the proposed work is to establish the biochemical mechanism and regulation of isoprene formation in the bacterial system, Bacillus subtilis. Specific objectives of the proposed work are the following: (A) to characterize the physiological regulation of isoprene formation in B. subtilis; (B) to characterize mutations in B. subtilis 168 that suppress isoprene formation, clone these genes, and determine how isoprene and isoprenoid carbon flow are regulated; and (C) to test ''overflow'' and ''signaling'' models for Bacillus isoprene formation. We are also pursuing the isolation and cloning of B. subtilis isoprene synthase, which we believe may be a regulatory enzyme.},
doi = {10.2172/792551},
url = {https://www.osti.gov/biblio/792551}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Mar 13 00:00:00 EST 2002},
month = {Wed Mar 13 00:00:00 EST 2002}
}