skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: TEM investigation of U{sup 6+} and Re{sup 7+} reduction by Desulfovibrio desulfuricans, a sulfate-reducing bacterium

Abstract

Uranium and its fission product Tc in aerobic environment will be in the forms of UO{sub 2}{sup 2+} and TcO{sub 4}{sup {minus}}. Reduced forms of tetravalent U and Tc are sparingly soluble. As determined by transmission electron microscopy, the reduction of uranyl acetate by immobilized cells of Desulfovibrio desulfuricans results in the production of black uraninite nanocrystals precipitated outside the cell. Some nanocrystals are associated with outer membranes of the cell as revealed from cross sections of these metabolic active sulfate-reducing bacteria. The nanocrystals have an average diameter of 5 nm and have anhedral shape. The reduction of Re{sup 7+} by cells of Desulfovibrio desulfuricans is fast in media containing H{sub 2} an electron donor, and slow in media containing lactic acid. It is proposed that the cytochrome in these cells has an important role in the reduction of uranyl and Re{sup 7+} is (a chemical analogue for Tc{sup 7+}) through transferring an electron from molecular hydrogen or lactic acid to the oxyions of UO{sub 2}{sup 2+} and TcO{sub 4}{sup {minus}}.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sponsoring Org.:
US Department of Energy (US)
OSTI Identifier:
752657
Report Number(s):
SAND2000-0688C
TRN: US0003152
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Conference
Resource Relation:
Conference: 1999 MRS Fall Meeting, Boston, MA (US), 11/28/2000--12/02/1999; Other Information: PBD: 14 Mar 2000
Country of Publication:
United States
Language:
English
Subject:
12 MANAGEMENT OF RADIOACTIVE WASTES, AND NON-RADIOACTIVE WASTES FROM NUCLEAR FACILITIES; URANIUM; TECHNETIUM; RHENIUM; URANYL COMPOUNDS; TECHNETIUM OXIDES; BIODEGRADATION; DESULFOVIBRIO; REDUCTION; PRECIPITATION; URANINITES; SOLUBILITY; WASTE WATER; DETOXIFICATION; RADIOACTIVE WASTE PROCESSING

Citation Formats

XU, HUIFANG, BARTON, LARRY L, CHOUDHURY, KEKA, ZHANG, PENGCHU, and WANG, YIFENG. TEM investigation of U{sup 6+} and Re{sup 7+} reduction by Desulfovibrio desulfuricans, a sulfate-reducing bacterium. United States: N. p., 2000. Web.
XU, HUIFANG, BARTON, LARRY L, CHOUDHURY, KEKA, ZHANG, PENGCHU, & WANG, YIFENG. TEM investigation of U{sup 6+} and Re{sup 7+} reduction by Desulfovibrio desulfuricans, a sulfate-reducing bacterium. United States.
XU, HUIFANG, BARTON, LARRY L, CHOUDHURY, KEKA, ZHANG, PENGCHU, and WANG, YIFENG. 2000. "TEM investigation of U{sup 6+} and Re{sup 7+} reduction by Desulfovibrio desulfuricans, a sulfate-reducing bacterium". United States. https://www.osti.gov/servlets/purl/752657.
@article{osti_752657,
title = {TEM investigation of U{sup 6+} and Re{sup 7+} reduction by Desulfovibrio desulfuricans, a sulfate-reducing bacterium},
author = {XU, HUIFANG and BARTON, LARRY L and CHOUDHURY, KEKA and ZHANG, PENGCHU and WANG, YIFENG},
abstractNote = {Uranium and its fission product Tc in aerobic environment will be in the forms of UO{sub 2}{sup 2+} and TcO{sub 4}{sup {minus}}. Reduced forms of tetravalent U and Tc are sparingly soluble. As determined by transmission electron microscopy, the reduction of uranyl acetate by immobilized cells of Desulfovibrio desulfuricans results in the production of black uraninite nanocrystals precipitated outside the cell. Some nanocrystals are associated with outer membranes of the cell as revealed from cross sections of these metabolic active sulfate-reducing bacteria. The nanocrystals have an average diameter of 5 nm and have anhedral shape. The reduction of Re{sup 7+} by cells of Desulfovibrio desulfuricans is fast in media containing H{sub 2} an electron donor, and slow in media containing lactic acid. It is proposed that the cytochrome in these cells has an important role in the reduction of uranyl and Re{sup 7+} is (a chemical analogue for Tc{sup 7+}) through transferring an electron from molecular hydrogen or lactic acid to the oxyions of UO{sub 2}{sup 2+} and TcO{sub 4}{sup {minus}}.},
doi = {},
url = {https://www.osti.gov/biblio/752657}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 14 00:00:00 EST 2000},
month = {Tue Mar 14 00:00:00 EST 2000}
}

Conference:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share: