TEM investigation of U{sup 6+} and Re{sup 7+} reduction by Desulfovibrio desulfuricans, a sulfate-reducing bacterium
Abstract
Uranium and its fission product Tc in aerobic environment will be in the forms of UO{sub 2}{sup 2+} and TcO{sub 4}{sup {minus}}. Reduced forms of tetravalent U and Tc are sparingly soluble. As determined by transmission electron microscopy, the reduction of uranyl acetate by immobilized cells of Desulfovibrio desulfuricans results in the production of black uraninite nanocrystals precipitated outside the cell. Some nanocrystals are associated with outer membranes of the cell as revealed from cross sections of these metabolic active sulfate-reducing bacteria. The nanocrystals have an average diameter of 5 nm and have anhedral shape. The reduction of Re{sup 7+} by cells of Desulfovibrio desulfuricans is fast in media containing H{sub 2} an electron donor, and slow in media containing lactic acid. It is proposed that the cytochrome in these cells has an important role in the reduction of uranyl and Re{sup 7+} is (a chemical analogue for Tc{sup 7+}) through transferring an electron from molecular hydrogen or lactic acid to the oxyions of UO{sub 2}{sup 2+} and TcO{sub 4}{sup {minus}}.
- Authors:
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Sponsoring Org.:
- US Department of Energy (US)
- OSTI Identifier:
- 752657
- Report Number(s):
- SAND2000-0688C
TRN: US0003152
- DOE Contract Number:
- AC04-94AL85000
- Resource Type:
- Conference
- Resource Relation:
- Conference: 1999 MRS Fall Meeting, Boston, MA (US), 11/28/2000--12/02/1999; Other Information: PBD: 14 Mar 2000
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 12 MANAGEMENT OF RADIOACTIVE WASTES, AND NON-RADIOACTIVE WASTES FROM NUCLEAR FACILITIES; URANIUM; TECHNETIUM; RHENIUM; URANYL COMPOUNDS; TECHNETIUM OXIDES; BIODEGRADATION; DESULFOVIBRIO; REDUCTION; PRECIPITATION; URANINITES; SOLUBILITY; WASTE WATER; DETOXIFICATION; RADIOACTIVE WASTE PROCESSING
Citation Formats
XU, HUIFANG, BARTON, LARRY L, CHOUDHURY, KEKA, ZHANG, PENGCHU, and WANG, YIFENG. TEM investigation of U{sup 6+} and Re{sup 7+} reduction by Desulfovibrio desulfuricans, a sulfate-reducing bacterium. United States: N. p., 2000.
Web.
XU, HUIFANG, BARTON, LARRY L, CHOUDHURY, KEKA, ZHANG, PENGCHU, & WANG, YIFENG. TEM investigation of U{sup 6+} and Re{sup 7+} reduction by Desulfovibrio desulfuricans, a sulfate-reducing bacterium. United States.
XU, HUIFANG, BARTON, LARRY L, CHOUDHURY, KEKA, ZHANG, PENGCHU, and WANG, YIFENG. 2000.
"TEM investigation of U{sup 6+} and Re{sup 7+} reduction by Desulfovibrio desulfuricans, a sulfate-reducing bacterium". United States. https://www.osti.gov/servlets/purl/752657.
@article{osti_752657,
title = {TEM investigation of U{sup 6+} and Re{sup 7+} reduction by Desulfovibrio desulfuricans, a sulfate-reducing bacterium},
author = {XU, HUIFANG and BARTON, LARRY L and CHOUDHURY, KEKA and ZHANG, PENGCHU and WANG, YIFENG},
abstractNote = {Uranium and its fission product Tc in aerobic environment will be in the forms of UO{sub 2}{sup 2+} and TcO{sub 4}{sup {minus}}. Reduced forms of tetravalent U and Tc are sparingly soluble. As determined by transmission electron microscopy, the reduction of uranyl acetate by immobilized cells of Desulfovibrio desulfuricans results in the production of black uraninite nanocrystals precipitated outside the cell. Some nanocrystals are associated with outer membranes of the cell as revealed from cross sections of these metabolic active sulfate-reducing bacteria. The nanocrystals have an average diameter of 5 nm and have anhedral shape. The reduction of Re{sup 7+} by cells of Desulfovibrio desulfuricans is fast in media containing H{sub 2} an electron donor, and slow in media containing lactic acid. It is proposed that the cytochrome in these cells has an important role in the reduction of uranyl and Re{sup 7+} is (a chemical analogue for Tc{sup 7+}) through transferring an electron from molecular hydrogen or lactic acid to the oxyions of UO{sub 2}{sup 2+} and TcO{sub 4}{sup {minus}}.},
doi = {},
url = {https://www.osti.gov/biblio/752657},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 14 00:00:00 EST 2000},
month = {Tue Mar 14 00:00:00 EST 2000}
}