Regulation of alcohol fermentation by Escherichia coli
Abstract
The purpose of this project is to elucidate the way in which the fermentative synthesis of ethanol is regulated in the facultative anaerobe Escherichia coli. We are also investigating the control of other genes required for fermentation and anaerobic growth. We have isolated both structural and regulatory mutations affecting the expression of alcohol dehydrogenase, the enzyme responsible for the final step in alcohol synthesis. Some of these regulatory mutations also affect other anaerobically induced genes. The adh gene has been cloned and sequenced. The ADH protein is one of the largest highly expressed proteins in E. coli and requires approximately 2700bp of DNA for its cloning sequence. We have also isolated mutations affecting the fermentative lactate dehydrogenase. In consequence it is now possible to construct E. coli strains defective in the production of any one or more of their normal fermentation products (i.e. formate, acetate, lactate, ethanol and succinate). The factors affecting the ratio of fermentation products are being investigated by in vivo NMR spectroscopy.
- Authors:
- Publication Date:
- Research Org.:
- Southern Illinois Univ., Carbondale, IL (United States). Dept. of Microbiology
- Sponsoring Org.:
- USDOE; USDOE, Washington, DC (United States)
- OSTI Identifier:
- 7279319
- Report Number(s):
- DOE/ER/13941-2
ON: DE92016870
- DOE Contract Number:
- FG02-88ER13941
- Resource Type:
- Technical Report
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; ALCOHOL DEHYDROGENASE; DNA SEQUENCING; ESCHERICHIA COLI; FERMENTATION; GENE REGULATION; LACTATE DEHYDROGENASE; PROGRESS REPORT; BACTERIA; BIOCONVERSION; DOCUMENT TYPES; ENZYMES; HEMIACETAL DEHYDROGENASES; MICROORGANISMS; ORGANIC COMPOUNDS; OXIDOREDUCTASES; PROTEINS; STRUCTURAL CHEMICAL ANALYSIS; 550200* - Biochemistry; 550700 - Microbiology
Citation Formats
Clark, D P. Regulation of alcohol fermentation by Escherichia coli. United States: N. p., 1989.
Web. doi:10.2172/7279319.
Clark, D P. Regulation of alcohol fermentation by Escherichia coli. United States. https://doi.org/10.2172/7279319
Clark, D P. 1989.
"Regulation of alcohol fermentation by Escherichia coli". United States. https://doi.org/10.2172/7279319. https://www.osti.gov/servlets/purl/7279319.
@article{osti_7279319,
title = {Regulation of alcohol fermentation by Escherichia coli},
author = {Clark, D P},
abstractNote = {The purpose of this project is to elucidate the way in which the fermentative synthesis of ethanol is regulated in the facultative anaerobe Escherichia coli. We are also investigating the control of other genes required for fermentation and anaerobic growth. We have isolated both structural and regulatory mutations affecting the expression of alcohol dehydrogenase, the enzyme responsible for the final step in alcohol synthesis. Some of these regulatory mutations also affect other anaerobically induced genes. The adh gene has been cloned and sequenced. The ADH protein is one of the largest highly expressed proteins in E. coli and requires approximately 2700bp of DNA for its cloning sequence. We have also isolated mutations affecting the fermentative lactate dehydrogenase. In consequence it is now possible to construct E. coli strains defective in the production of any one or more of their normal fermentation products (i.e. formate, acetate, lactate, ethanol and succinate). The factors affecting the ratio of fermentation products are being investigated by in vivo NMR spectroscopy.},
doi = {10.2172/7279319},
url = {https://www.osti.gov/biblio/7279319},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1989},
month = {Sun Jan 01 00:00:00 EST 1989}
}