Enzymology of acetone-butanol-isopropanol formation
The objectives of the project are to elucidate first the molecular properties of solvent-forming enzymes and then to apply the information gained from the enzymological study to elucidate the control mechanisms for the solvent-producing pathways and the expression of solvent-production genes. Our research primarily involves two strains of Clostridium beijerinckii (Clostridium butylicum): C. beijerinckii NRRL B593 which produces isopropanol in addition to acetone, n-butanol, and ethanol, and C. beijerinckii NRRL B592 which produces acetone, n-butanol and ethanol, but not isopropanol. More recently, we started to include another solvent-producing organism, Bacillus macerans, in our study. B. macerans produces a high level of acetone and ethanol under anaerobic conditions. Because B. macerans does not produce butyric acid and butanol, it has a less complex metabolic pathway than that of C. acetobutylicum and C. beijerinckii. This simpler metabolic pathway serves as a useful system for comparison in our study of the enzymology and regulation of solvent formation.
- Research Organization:
- Virginia Polytechnic Inst. and State Univ., Blacksburg, VA (USA)
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- FG05-85ER13368
- OSTI ID:
- 7147408
- Report Number(s):
- DOE/ER/13368-6; ON: DE90007660
- Country of Publication:
- United States
- Language:
- English
Similar Records
Enzymology of acetone-butanol-isopropanol formation
Enzymology of acetone-butanol-isopropanol formation. Progress report, January 1, 1991--December 31, 1991