Relationship of the superoxide dismutase genes, sodA and sodB, to the iron uptake (/ital fur/) regulon in /ital Escherichia coli/ K-12

Conference · Fri Jan 01 00:00:00 EST 1988

OSTI ID:6954511

Niederhoffer, E C; Naranjo, C M; Fee, J A

Expression of sodA, as indicated by MnSod activity is normal in /ital fur/ mutants. This suggests that sodA is not a member of the /ital fur/ regulon and that the putative Fe-binding, regulatory protein of sodA, suggested by Moody and Hassan is not the Fur protein. by contrast, expression of sodB, as indicated by FeSod activity, is completely blocked in /ital fur/ mutants and the effect is restored by transformation with a plasmid having a normal /ital fur/ locus. The observations suggest that Fur, either directly or indirectly, controls SodB biosynthesis. Additional observations are described which indicate that SodB and Fur act together in a complicated fashion to control the biosynthesis of enterobactin. 26 refs., 3 tabs.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 6954511
• Report Number(s): LA-UR-88-1812; CONF-8804139-2; ON: DE88014461
• Resource Relation: Conference: UCLA symposia, Frisco, CO, USA, 10 Apr 1988; Other Information: Portions of this document are illegible in microfiche products
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENE OPERONS
PORINS
GENE REGULATION
SUPEROXIDE DISMUTASE
ESCHERICHIA COLI
GENE MUTATIONS
IRON
MEMBRANE TRANSPORT
BACTERIA
ELEMENTS
ENZYMES
MEMBRANE PROTEINS
METALS
MICROORGANISMS
MUTATIONS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
TRANSITION ELEMENTS
550200* - Biochemistry
550400 - Genetics