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Title: Annual progress report, December 1, 1979-November 30, 1980

Abstract

Structure and function in photosynthetic membranes and their components continue to be studied, principally with Rhodopseudomonas sphaeroides. We have discovered how to remove bacteriochlorophyll (bchl) from a specific binding site in the antenna pigment-protein complex B850, and to replace the bchl without denaturing the complex. This has never before been achieved for any photosynthetic chlorophyllprotein complex. It opens a substantial vista of experiments designed to elucidate the nature of the binding site. Our analyses of the B850 and B875 complexes cast doubt on the accepted ratios of bchl components, carotenoids and protein and hence on models based on these ratios. We have shown that Rp. sphaeroides contains a specific second quinone (Q/sub b/) in contact with the primary photochemical electron acceptor (Q/sub a/) and distinct from the molecules of a larger pool of quinone. We have resolved conflicting reports about the polypeptide composition of reaction centers from Rhodopseudomonas viridis. We have new data on the orientation, relative to the photosynthetic membrane, of cytochrome 552 in Rp. viridis and of the shifting component of carotenoid in Rp. sphaeroides. We have constructed and are using a new circular dichroism spectrometer.

Authors:
Publication Date:
Research Org.:
Cornell Univ., Ithaca, NY (United States)
OSTI Identifier:
6702659
Report Number(s):
COO-3162-53; COO-3162-54(App.B)
DOE Contract Number:  
AS02-76ER03162
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELL MEMBRANES; MOLECULAR STRUCTURE; PHOTOSYNTHESIS; RESEARCH PROGRAMS; CAROTENOIDS; CHEMICAL BONDS; CHLOROPHYLL; COMPLEXES; CYTOCHROMES; PHOTOSYNTHETIC BACTERIA; PROTEINS; RHODOPSEUDOMONAS; BACTERIA; CARBOXYLIC ACIDS; CELL CONSTITUENTS; CHEMICAL REACTIONS; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYDROCARBONS; MEMBRANES; MICROORGANISMS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHOTOCHEMICAL REACTIONS; PHYTOCHROMES; PIGMENTS; PORPHYRINS; SYNTHESIS; TERPENES; 550300* - Cytology

Citation Formats

Clayton, R K. Annual progress report, December 1, 1979-November 30, 1980. United States: N. p., 1980. Web. doi:10.2172/6702659.
Clayton, R K. Annual progress report, December 1, 1979-November 30, 1980. United States. https://doi.org/10.2172/6702659
Clayton, R K. 1980. "Annual progress report, December 1, 1979-November 30, 1980". United States. https://doi.org/10.2172/6702659. https://www.osti.gov/servlets/purl/6702659.
@article{osti_6702659,
title = {Annual progress report, December 1, 1979-November 30, 1980},
author = {Clayton, R K},
abstractNote = {Structure and function in photosynthetic membranes and their components continue to be studied, principally with Rhodopseudomonas sphaeroides. We have discovered how to remove bacteriochlorophyll (bchl) from a specific binding site in the antenna pigment-protein complex B850, and to replace the bchl without denaturing the complex. This has never before been achieved for any photosynthetic chlorophyllprotein complex. It opens a substantial vista of experiments designed to elucidate the nature of the binding site. Our analyses of the B850 and B875 complexes cast doubt on the accepted ratios of bchl components, carotenoids and protein and hence on models based on these ratios. We have shown that Rp. sphaeroides contains a specific second quinone (Q/sub b/) in contact with the primary photochemical electron acceptor (Q/sub a/) and distinct from the molecules of a larger pool of quinone. We have resolved conflicting reports about the polypeptide composition of reaction centers from Rhodopseudomonas viridis. We have new data on the orientation, relative to the photosynthetic membrane, of cytochrome 552 in Rp. viridis and of the shifting component of carotenoid in Rp. sphaeroides. We have constructed and are using a new circular dichroism spectrometer.},
doi = {10.2172/6702659},
url = {https://www.osti.gov/biblio/6702659}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1980},
month = {Tue Jan 01 00:00:00 EST 1980}
}