Molecular mapping of chromosomes 17 and X
The basic aims of this project are the construction of high density genetic maps of chromosomes 17 and X and the utilization of these maps for the subsequent isolation of a set of physically overlapping DNA segment clones. The strategy depends on the utilization of chromosome specific libraries of small (1--15 kb) segments from each of the two chromosomes. Since the time of submission of our previous progress report, we have refined the genetic map of markers which we had previously isolated for chromosome 17. We have completed our genetic mapping in CEPH reference and NF1 families of 15 markers in the pericentric region of chromosome 17. Physical mapping results with three probes, were shown be in very close genetic proximity to the NF1 gene, with respect to two translocation breakpoints which disrupt the activity of the gene. All three of the probes were found to lie between the centromere and the most proximal translocation breakpoint, providing important genetic markers proximal to the NF1 gene. Our primary focus has shifted to the X chromosome. We have isolated an additional 30 polymorphic markers, bringing the total number we have isolated to over 80. We have invested substantial effort in characterizing the polymorphisms at each of these loci and constructed plasmid subclones which reveal the polymorphisms for nearly all of the loci. These subclones are of practical value in that they produce simpler and stronger patterns on human genomic Southern blots, thus improving the efficiency of the genetic mapping experiments. These subclones may also be of value for deriving DNA sequence information at each locus, necessary for establishing polymerase chain reaction primers specific for each locus. Such information would allow the use of each locus as a sequence tagged site.
- Research Organization:
- Utah Univ., Salt Lake City, UT (United States). Dept. of Medical Informatics
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-88ER60689
- OSTI ID:
- 6697096
- Report Number(s):
- DOE/ER/60689-2; ON: DE93009366
- Country of Publication:
- United States
- Language:
- English
Similar Records
Molecular mapping of chromosomes 17 and X
Molecular mapping of chromosomes 17 and X. Progress report
Related Subjects
HUMAN CHROMOSOME 17
GENETIC MAPPING
HUMAN X CHROMOSOME
RFLPS
DNA-CLONING
HEREDITARY DISEASES
PROGRESS REPORT
CHROMOSOMES
CLONING
DISEASES
DNA HYBRIDIZATION
DOCUMENT TYPES
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
X CHROMOSOME
550400* - Genetics
550200 - Biochemistry