Molecular mapping of chromosomes 17 and X
Progress toward the construction of high density genetic maps of chromosomes 17 and X has been made by isolating and characterizing a relatively large set of polymorphic probes for each chromosome and using these probes to construct genetic maps. We have mapped the same polymorphic probes against a series of chromosome breakpoints on X and 17. The probes could be assigned to over 30 physical intervals on the X chromosome and 7 intervals on 17. In many cases, this process resulted in improved characterization of the relative locations of the breakpoints with respect to each other and the definition of new physical intervals. The strategy for isolation of the polymorphic clones utilized chromosome specific libraries of 1--15 kb segments from each of the two chromosomes. From these libraries, clones were screened for those detecting restriction fragment length polymorphisms. The markers were further characterized, the chromosomal assignments confirmed and in most cases segments of the original probes were subcloned into plasmids to produce probes with improved signal to noise ratios for use in the genetic marker studies. The linkage studies utilize the CEPH reference families and other well-characterized families in our collection which have been used for genetic disease linkage work. Preliminary maps and maps of portions of specific regions of 17 and X are provided. We have nearly completed a map of the 1 megabase Mycoplasma arthritidis genome by applying these techniques to a lambda phage library of its genome. We have found bit mapping to be an efficient means to organize a contiguous set of overlapping clones from a larger genome.
- Research Organization:
- Utah Univ., Salt Lake City, UT (United States). Dept. of Medical Informatics
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-88ER60689
- OSTI ID:
- 6659529
- Report Number(s):
- DOE/ER/60689-3; ON: DE93010918
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
COLLAGEN
GENE MUTATIONS
GENETIC MAPPING
OPTIMIZATION
HUMAN CHROMOSOME 17
HUMAN X CHROMOSOME
MYCOPLASMA
ELECTROPHORESIS
HEREDITARY DISEASES
PROGRESS REPORT
RFLPS
RHEUMATIC DISEASES
CHROMOSOMES
DISEASES
DOCUMENT TYPES
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
MAPPING
MICROORGANISMS
MUTATIONS
ORGANIC COMPOUNDS
PROTEINS
SCLEROPROTEINS
SKELETAL DISEASES
X CHROMOSOME
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