Reconstitution of halorhodopsin
Halobacterium halobium contains a family of retinal-bound proteins: bacteriorhodopsin (bR) which mediates phototrophic growth as a light-riven proton pump, halorhodopsin (hR) which is a light-driven chloride pump, and one or more sensory rhodopsins (sR) which mediate a phototactic response. Two-dimensional crystallization of halorhodopsin has been attempted though the reconstitution of purified halorhodopsin with purple membrane lipid for electron microscopy work. The first important step for crystallization is to get a homogeneous protein which is pure and not denatured. Homogeneous halorhodopsin has been obtained by a modification of existing purification methods. Some nice looking membrane patches which have the same density as purple membrane have been obtained. But unfortunately, they are not crystalline. The procedure of hR reconstitution is described in detail and some other strategies to induce the protein crystal in the reconstituted membrane are discussed in this dissertation. 76 refs., 20 figs., 6 tabs.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- DOE/ER; DOHHS
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 6374468
- Report Number(s):
- LBL-28624; ON: DE91001791; CNN: GM36884
- Resource Relation:
- Other Information: Thesis (Ph.D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
PROTEINS
ELECTRON MICROSCOPY
RHODOPSIN
CRYSTALLIZATION
ATP
BACTERIA
BRINES
DICHROISM
PHOTOCHEMISTRY
PURIFICATION
SPECTROSCOPY
CHEMISTRY
MICROORGANISMS
MICROSCOPY
NUCLEOTIDES
ORGANIC COMPOUNDS
PHASE TRANSFORMATIONS
PIGMENTS
550200* - Biochemistry
400102 - Chemical & Spectral Procedures