Isolation, separation, and characterization of epithelial and connective cells from rat palate

Thesis/Dissertation · Mon Jan 01 00:00:00 EST 1979

DOI:https://doi.org/10.2172/6205574· OSTI ID:6205574

Terranova, Victor Paul ^[1]

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1. Univ. of Rochester, NY (United States)

Epithelial and connective tissue cells were isolated from rat palate by sequential collagenase, hyaluronidase and trypsin digestion of the extracellular matrix. Differences between the two populations were noted with respect to total cell protein, total cell water, proline uptake and incorporation, percent collagen synthesized, effects of parathyroid hormone, metabolism of D-valine and cell density. Basal epithelial cells were subsequently separated from the heterogeneous epithelial cell population on shallow linear density gradients by velocity centrifugation. The type of collagen synthesized by the basal epithelial cells was compared to the type of collagen synthesized by the connective tissue cells by means of labeled amino acid incorporation ratios. Cells isolated from the epithelial and connective tissue were compared. From these studies it can be concluded that epithelial and connective tissue cells can be isolated from rat palate as viable and distinct populations with respect to the biochemical parameters examined. Furthermore, subpopulations can be separated and biochemically characterized.

• Research Organization: Univ. of Rochester, NY (United States). Dept. of Radiation Biology and Biophysics
• Sponsoring Organization: USDOE
• DOE Contract Number: EY-76-C-02-3490
• OSTI ID: 6205574
• Report Number(s): UR-3490-1524
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ANIMAL CELLS
CHEMICAL COMPOSITION
ORAL CAVITY
HISTOLOGY
BIOSYNTHESIS
COLLAGEN
CONNECTIVE TISSUE
EPITHELIUM
EXPERIMENTAL DATA
GRAPHS
ISOLATED VALUES
METABOLISM
PARATHORMONE
PROTEINS
WATER
BODY
DATA
DATA FORMS
DIGESTIVE SYSTEM
HORMONES
HYDROGEN COMPOUNDS
INFORMATION
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
SCLEROPROTEINS
SYNTHESIS
TISSUES
550200* - Biochemistry
550300 - Cytology